BMRB Entry 15113

Title:
Solution Structure of the Rous Sarcoma Virus Nucleocapsid Protein:muPsi RNA Packaging Signal Complex   PubMed: 17070546
Deposition date:
2007-01-24
Original release date:
2007-03-05
Authors:
Zhou, J.; Summers, M.
Citation:

Citation: Zhou, J.; Bean, R.; Vogt, V.; Summers, M.. "Solution Structure of the Rous Sarcoma Virus Nucleocaspid Protein:muPsi RNA Packaging Signal Complex"  J. Mol. Biol. 365, 453-467 (2007).

Assembly members:

Assembly members:
ZN, non-polymer, Formula weight is not available
muPsi_RNA, polymer, 75 residues, Formula weight is not available
Nucleocapsid_(NC)_Protein, polymer, 61 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 11886   Superkingdom: Viruses   Kingdom: not available   Genus/species: Alpharetrovirus Rous sarcoma virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-1

Experimental source:

Natural source:   Common Name: not available   Taxonomy ID: 11886   Superkingdom: Viruses   Kingdom: not available   Genus/species: Alpharetrovirus Rous sarcoma virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-1

Data sets:
Data typeCount
13C chemical shifts88
1H chemical shifts567

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Zn1
2muPsi RNA2
3NC protein3

Entities:

Entity 1, Zn - Formula weight is not available

1   ZN

Entity 2, muPsi RNA 75 residues - Formula weight is not available

1   CUGCCCUCAU
2   CCGUCUCGCU
3   UAUUCGGGGA
4   GCGGACGAUG
5   ACCCUAGUAG
6   AGGGGGCUGC
7   GGCUUAGGAG
8   GGCAG

Entity 3, NC protein 61 residues - Formula weight is not available

1   GLYARGALAARGGLYLEUCYSTYRTHRCYS
2   GLYSERPROGLYHISTYRGLNALAGLNCYS
3   PROLYSLYSARGLYSSERGLYASNSERARG
4   GLUARGCYSGLNLEUCYSASNGLYMETGLY
5   HISASNALALYSGLNCYSARGLYSARGASP
6   GLYASNGLNGLYGLNARGPROGLYLYSGLY
7   LEU

Samples:

sample_1: muPsi RNA, G, C, U 2H-enriched, 1.0 mM; Nucleocapsid (NC) Protein 1.0 mM; Tris-d11-HCl 5 mM; NaCL 5 mM; ZnCl2 0.1 mM; D2O 100%

sample_2: muPsi RNA, A, C, U 2H-enriched, 1.2 mM; Nucleocapsid (NC) Protein 1.2 mM; Tris-d11-HCl 5 mM; NaCL 5 mM; ZnCl2 0.1 mM; D2O 100%

sample_3: muPsi RNA, A, G, C 2H-enriched, 0.8 mM; Nucleocapsid (NC) Protein 0.8 mM; Tris-d11-HCl 5 mM; NaCL 5 mM; ZnCl2 0.1 mM; D2O 100%

sample_4: muPsi RNA, A, G, U 2H-enriched, 0.8 mM; Nucleocapsid (NC) Protein 0.8 mM; Tris-d11-HCl 5 mM; NaCL 5 mM; ZnCl2 0.1 mM; D2O 100%

sample_5: muPsi RNA 1.2 mM; Nucleocapsid (NC) Protein 1.2 mM; Tris-d11-HCl 5 mM; NaCL 5 mM; ZnCl2 0.1 mM; D2O 100%

sample_6: muPsi RNA 1.2 mM; Nucleocapsid (NC) Protein, [U-98% 13C; U-98% 15N], 1.2 mM; Tris-d11-HCl 5 mM; NaCL 5 mM; ZnCl2 0.1 mM; D2O 100%

sample_conditions_1: ionic strength: 0.0053 M; pH: 7.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1isotropicsample_conditions_1
2D NOESYsample_2isotropicsample_conditions_1
2D NOESYsample_3isotropicsample_conditions_1
2D NOESYsample_4isotropicsample_conditions_1
2D NOESYsample_5isotropicsample_conditions_1
3D_13C-separated_NOESYsample_6isotropicsample_conditions_1

Software:

xwinnmr, BRUKER - collection

NMRPipe, Delaglio, F., Grzesiek. S., Vuister, G.W., Zhu, G., Pfeifer, J., & Bax, A. - processing

NMRView v5, Johnson, B.A., Blevins, R.A. - data analysis

CYANA v2.1, Guntert, P., Mumenthaler, C. & Wuthrich, K. - refinement

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
EMBL CAA24512
GB AAB59932 AAB59933 AAC82560 AAC82561 ABD83647
REF NP_056886 NP_056887 NP_955610
SP P03322 P03354