BMRB Entry 15103

Name: Structure of NOWA cysteine rich domain 6
Published: 2007-10-17

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PDB ID: 2nx6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15103
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of NOWA cysteine rich domain 6

Deposition date:

2007-01-22

Original release date:

2007-10-17

Authors:

Meier, S.; Jensen, P.; Adamczyk, P.; Bachinger, H.; Holstein, T.; Engel, J.; Ozbek, S.; Grzesiek, S.

Citation:

Citation: Meier, S.; Jensen, P.; Adamczyk, P.; Bachinger, H.; Holstein, T.; Engel, J.; Oezbek, S.; Grzesiek, S.. "Sequence-structure and structure-function analysis in cysteine-rich domains forming the ultrastable nematocyst wall" J. Mol. Biol. 368, 718-728 (2007).
PubMed: 17362991

Assembly members:

Assembly members:
Nematocyst_outer_wall_antigen, polymer, 27 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Hydra vulgaris Taxonomy ID: 6087 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Hydra vulgaris

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEV2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nematocyst_outer_wall_antigen: GSSSCPQFPSCSPSCAPQCS QQCCQQP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	21
1H chemical shifts	21

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	nw6	1

Entities:

Entity 1, nw6 27 residues - Formula weight is not available

1

GLY

SER

CYS

PRO

GLN

PHE

PRO

SER

2

CYS

SER

PRO

SER

CYS

ALA

PRO

GLN

CYS

SER

3

GLN

CYS

GLN

PRO

Samples:

sample: Nematocyst outer wall antigen, [U-100% 15N], 0.5 mM; D2O, [U-2H], 5%; potassium phosphate 5 mM

sample_conditions_1: ionic strength: 20 mM; pH: 5.5; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	sample	isotropic	sample_conditions_1
2D TOCSY	sample	isotropic	sample_conditions_1
3D 15N-separated ROESY	sample	isotropic	sample_conditions_1
15N HSQC	sample	isotropic	sample_conditions_1

Software:

CNS v1.0, Brunger A. T. et al - refinement

NMRPipe v2.1, Delaglio et al - processing

xwinnmr v3.5, Bruker Biospin - collection

PIPP v4.3.2, Garrett D. et al - data analysis

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DRX 600 MHz