BMRB Entry 15101
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15101
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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Citation: Seo, Min-Duk; Park, Sung Jean; Kim, Hyun-Jung; Lee, Bong-Jin. "Solution structure of hypothetical protein, HP0495 (Y495_HELPY) from Helicobacter pylori" Proteins 67, 1189-1192 (2007).
PubMed: 17373705
Assembly members:
HP0495, polymer, 86 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a
Entity Sequences (FASTA):
HP0495: MPSDSKKPTIIYPCLWDYRV
IMTTKDTSTLKELLETYQRP
FKLEFKNTSKNAKFYSFNVS
MEVSNESERNEIFQKISQLD
KVVQTL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 246 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 159 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HP0495 | 1 |
Entities:
Entity 1, HP0495 86 residues - Formula weight is not available
| 1 | MET | PRO | SER | ASP | SER | LYS | LYS | PRO | THR | ILE | ||||
| 2 | ILE | TYR | PRO | CYS | LEU | TRP | ASP | TYR | ARG | VAL | ||||
| 3 | ILE | MET | THR | THR | LYS | ASP | THR | SER | THR | LEU | ||||
| 4 | LYS | GLU | LEU | LEU | GLU | THR | TYR | GLN | ARG | PRO | ||||
| 5 | PHE | LYS | LEU | GLU | PHE | LYS | ASN | THR | SER | LYS | ||||
| 6 | ASN | ALA | LYS | PHE | TYR | SER | PHE | ASN | VAL | SER | ||||
| 7 | MET | GLU | VAL | SER | ASN | GLU | SER | GLU | ARG | ASN | ||||
| 8 | GLU | ILE | PHE | GLN | LYS | ILE | SER | GLN | LEU | ASP | ||||
| 9 | LYS | VAL | VAL | GLN | THR | LEU |
Samples:
sample_1: HP0495, [U-99% 13C; U-99% 15N], 1.2 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 15190 |
| PDB | |
| GB | AAD07569 ADU82921 AFV41714 AFV43308 AFV44901 |
| REF | NP_207292 WP_001138777 WP_001138778 |
| SP | O25237 |
| AlphaFold | O25237 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks