BMRB Entry 15094

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15094

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Title:
1H, 13C, and 15N Chemical Shift Assignments for CutA1 from E. coli
Deposition date:
2007-01-04
Original release date:
2007-10-17
Authors:
Bertini, Ivano; Jimenez, Beatriz; Pierattelli, Roberta
Citation:

Citation: Bertini, Ivano; Jimenez, Beatriz; Pierattelli, Roberta; Wedd, Anthony; Xiao, Zhiguang. "Protonless (13)C direct detection NMR: characterization of the 37 kiloDalton trimeric protein CutA1"  Proteins 70, 1196-1205 (2008).
PubMed: 17847095

Assembly members:

Assembly members:
CutA1, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CutA1: MLDEKSSNTASVVVLCTAPD EATAQDLAAKVLAEKLAACA TLIPGATSLYYWEGKLEQEY EVQMILKTTVSHQQALLECL KSHHPYQTPELLVLPVTHGD TDYLSWLNASLR

Data sets:
Data typeCount
13C chemical shifts383
15N chemical shifts119
1H chemical shifts228

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CutA1, chain 11
2CutA1, chain 21
3CutA1, chain 31

Entities:

Entity 1, CutA1, chain 1 112 residues - Formula weight is not available

1   METLEUASPGLULYSSERSERASNTHRALA
2   SERVALVALVALLEUCYSTHRALAPROASP
3   GLUALATHRALAGLNASPLEUALAALALYS
4   VALLEUALAGLULYSLEUALAALACYSALA
5   THRLEUILEPROGLYALATHRSERLEUTYR
6   TYRTRPGLUGLYLYSLEUGLUGLNGLUTYR
7   GLUVALGLNMETILELEULYSTHRTHRVAL
8   SERHISGLNGLNALALEULEUGLUCYSLEU
9   LYSSERHISHISPROTYRGLNTHRPROGLU
10   LEULEUVALLEUPROVALTHRHISGLYASP
11   THRASPTYRLEUSERTRPLEUASNALASER
12   LEUARG

Samples:

sample_1: CutA1, [U-83% 13C; U-98% 15N], 3.5 mM

sample_2: CutA1, [U-98% 15N], 3.5 mM

sample_conditions_1: ionic strength: 0.04 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D CACOsample_1isotropicsample_conditions_1
2D CONsample_1isotropicsample_conditions_1
2D CBCACOsample_1isotropicsample_conditions_1

Software:

CARA v1.5, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAB38541 BAE78139 BAG66863 BAG79960 BAI28441
EMBL CAA54780 CAA85374 CAP78655 CAQ34486 CAR01113
GB AAA97036 AAC77097 AAG59336 AAN45709 AAN83641
REF NP_313145 NP_418560 NP_710002 WP_000883338 WP_000883339
SP A7ZV06 A8A7N3 B1ITR1 B1LQF8 B1XD17
AlphaFold A7ZV06 A8A7N3 B1ITR1 B1LQF8 B1XD17

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks