BMRB Entry 15093

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15093

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Title:
preB ApoAI backbone assignment
Deposition date:
2007-01-04
Original release date:
2007-06-26
Authors:
Ren, Xuefeng; Yang, Yunhuang; Wang, Jianjun
Citation:

Citation: Ren, Xuefeng; Yang, Yunhuang; Tracey, Neville; Hoyt, David; Sparks, Daniel; Wang, Jianjun. "A complete backbone spectral assignment of human apolipoprotein AI on a 38 kDa prebetaHDL (Lp1-AI) particle"  Biomol. NMR Assignments 1, 69-71 (2007).
PubMed: 19636829

Assembly members:

Assembly members:
preB_ApoAI, polymer, 243 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-30(+)a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
preB_ApoAI: DEPPQSPWDRVKDLATVYVD VLKDSGRDYVSQFEGSALGK QLNLKLLDNWDSVTSTFSKL REQLGPVTQEFWDNLEKETE GLRQEMSKDLEEVKAKVQPY LDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEK LSPLGEEMRDRARAHVDALR THLAPYSDELRQRLAARLEA LKENGGARLAEYHAKATEHL STLSEKAKPALEDLRQGLLP VLESFKVSFLSALEEYTKKL NTQ

Data sets:
Data typeCount
13C chemical shifts458
15N chemical shifts211
1H chemical shifts212

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1preB_ApoAI1

Entities:

Entity 1, preB_ApoAI 243 residues - Formula weight is not available

1   ASPGLUPROPROGLNSERPROTRPASPARG
2   VALLYSASPLEUALATHRVALTYRVALASP
3   VALLEULYSASPSERGLYARGASPTYRVAL
4   SERGLNPHEGLUGLYSERALALEUGLYLYS
5   GLNLEUASNLEULYSLEULEUASPASNTRP
6   ASPSERVALTHRSERTHRPHESERLYSLEU
7   ARGGLUGLNLEUGLYPROVALTHRGLNGLU
8   PHETRPASPASNLEUGLULYSGLUTHRGLU
9   GLYLEUARGGLNGLUMETSERLYSASPLEU
10   GLUGLUVALLYSALALYSVALGLNPROTYR
11   LEUASPASPPHEGLNLYSLYSTRPGLNGLU
12   GLUMETGLULEUTYRARGGLNLYSVALGLU
13   PROLEUARGALAGLULEUGLNGLUGLYALA
14   ARGGLNLYSLEUHISGLULEUGLNGLULYS
15   LEUSERPROLEUGLYGLUGLUMETARGASP
16   ARGALAARGALAHISVALASPALALEUARG
17   THRHISLEUALAPROTYRSERASPGLULEU
18   ARGGLNARGLEUALAALAARGLEUGLUALA
19   LEULYSGLUASNGLYGLYALAARGLEUALA
20   GLUTYRHISALALYSALATHRGLUHISLEU
21   SERTHRLEUSERGLULYSALALYSPROALA
22   LEUGLUASPLEUARGGLNGLYLEULEUPRO
23   VALLEUGLUSERPHELYSVALSERPHELEU
24   SERALALEUGLUGLUTYRTHRLYSLYSLEU
25   ASNTHRGLN

Samples:

sample_1: preB_ApoAI, [U-13C; U-15N; U-2H], 1 mM; DSS 0.1 mM; sodium azide 1 mM; D2O 5%; DTT 95%; sodium phosphate 25 mM; sodium chloride 25 mM; EDTA 10 mM

sample_conditions_1: ionic strength: 0.08 M; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOCACBsample_1isotropicsample_conditions_1
3D 15N-NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian Unity 500 MHz
  • Varian UnityPlus 900 MHz

Related Database Links:

PDB
DBJ BAF84920 BAI46675
EMBL CAA25232 CAA25519 CAA26097 CAA30377
GB AAA35545 AAA51747 AAA62829 AAB59514 AAH05380
REF NP_000030 XP_001153383 XP_001153517 XP_003253233 XP_003253235
SP G3QY98 P02647 P0DJG0 P0DJG1 P0DM91
AlphaFold G3QY98 P02647 P0DJG0 P0DJG1 P0DM91

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks