BMRB Entry 15081

Name: U2 snRNA stem I from human
Published: 2007-02-02

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PDB ID: 2o32
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15081
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

U2 snRNA stem I from human

Deposition date:

2006-12-15

Original release date:

2007-02-02

Authors:

Sashital, Dipali; Venditti, Vincenzo; Angers, Cortney; Cornilescu, Gabriel; Butcher, Samuel

Citation:

Citation: Sashital, Dipali; Venditti, Vincenzo; Angers, Cortney; Cornilescu, Gabriel; Butcher, Samuel. "Structure and thermodynamics of a conserved U2 snRNA domain from yeast and human" RNA 13, 328-338 (2007).
PubMed: 17242306

Assembly members:

Assembly members:
Human_U2_stem_I, polymer, 20 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human_U2_stem_I: XCUCXXCCXUUUXGCUAAXA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	68
1H chemical shifts	124

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Human U2 Stem I	1

Entities:

Entity 1, Human U2 Stem I 20 residues - Formula weight is not available

1		PSU	C	U	C	OMG	OMG	C	C	PSU	U
2		U	U	OMG	G	C	U	A	A	OMG	A

Samples:

sample_1: Human U2 stem I 0.5 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_2: Human U2 stem I 0.5 mM; sodium chloride 50 mM; D2O 99.99%

sample_3: Human U2 stem I 0.5 mM; sodium chloride 50 mM; potassium phosphate 10 mM; Pf1 phage 17 w/v; D2O 99.99%

sample_conditions_1: pH: 7.0; temperature: 283 K

sample_conditions_2: pH: 7.0; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_2
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_2
2D 1H-13C HSQC	sample_3	anisotropic	sample_conditions_2
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_2

Software:

xwinnmr, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

Bruker DMX 750 MHz