BMRB Entry 15058

Name: 1H assignment of En-6
Published: 2007-10-16

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PDB ID: 2jms
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15058
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H assignment of En-6

Deposition date:

2006-11-29

Original release date:

2007-10-16

Authors:

Pedrini, Bill; Placzek, William; Koculi, Eda; Alimenti, Claudio; LaTerza, Antonietta; Luporini, Pierangelo; Wuthrich, Kurt

Citation:

Citation: Pedrini, Bill; Placzek, William; Koculi, Eda; Alimenti, Claudio; LaTerza, Antonietta; Luporini, Pierangelo; Wuthrich, Kurt. "Cold-adaptation in sea-water-borne signal proteins: Sequence and NMR structure of the pheromone En-6 from the Antarctic ciliate Euplotes nobilii" J. Mol. Biol. 372, 277-286 (2007).
PubMed: 17663000

Assembly members:

Assembly members:
En-6, polymer, 63 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Euplotes nobilii Taxonomy ID: 184062 Superkingdom: Eukaryota Kingdom: not available Genus/species: Euplotes nobilii

Experimental source:

Experimental source: Production method: purified from the natural source Host organism: Euplotes nobilii

Entity Sequences (FASTA):

Entity Sequences (FASTA):
En-6: TDPEEHFDPNTNCDYTNSQD AWDYCTNYIVNSSCGEICCN DCFDETGTGACRAQAFGNSC LNW

Data sets:

assigned_chemical_shifts
- EN6_NMR-STAR

Data type	Count
1H chemical shifts	344

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	En-6 monomer	1

Entities:

Entity 1, En-6 monomer 63 residues - Formula weight is not available

1

THR

ASP

PRO

GLU

HIS

PHE

ASP

PRO

ASN

2

THR

ASN

CYS

ASP

TYR

THR

ASN

SER

GLN

ASP

3

ALA

TRP

ASP

TYR

CYS

THR

ASN

TYR

ILE

VAL

4

ASN

SER

CYS

GLY

GLU

ILE

CYS

ASN

5

ASP

CYS

PHE

ASP

GLU

THR

GLY

THR

GLY

ALA

6

CYS

ARG

ALA

GLN

ALA

PHE

GLY

ASN

SER

CYS

7

LEU

ASN

TRP

Samples:

sample_1: En-6 1 ± 0.2 mM; sodium phosphate 20 ± 0.5 mM

sample_2: En-6 1 ± 0.2 mM; sodium phosphate 20 ± 0.5 mM

sample_3: En-6 1 ± 0.2 mM; sodium phosphate 20 ± 0.5 mM; DSS 1 ± 0.1 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
1D 1H	sample_3	isotropic	sample_conditions_1

Software:

CARA v1.5.3, Keller, R - chemical shift assignment

XWINNMR, Bruker Biospin - collection

ATNOS-CANDID v1.2, Guntert, Mumenthaler and Wuthrich, Herrmann, Guntert and Wuthrich - peak assignment, peak picking, structure solution

OPALp, Koradi, Billeter and Guntert - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - peak assignment, peak picking, structure solution

NMR spectrometers:

Bruker Avance 600 MHz

PDB	2JMS
GB	ABK15649