BMRB Entry 15057

Name: Solution Structrue of C-terminal Bromodomain of Brd4
Published: 2008-06-24

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15057

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structrue of C-terminal Bromodomain of Brd4

Deposition date:

2006-11-28

Original release date:

2008-06-24

Authors:

Liu, Y.; Wu, J.; Shi, Y.

Citation:

Citation: Liu, Y.; Wang, X.; Zhang, J.; Huang, H.; Ding, B.; Wu, J.; Shi, Y.. "Structural basis and binding properties of the second bromodomain of Brd4 with acetylated histone tails" Biochemistry 47, 6403-6417 (2008).
PubMed: 18500820

Assembly members:

Assembly members:
Bromodomain-containing_protein_4, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Bromodomain-containing_protein_4: EQLKCCSGILKEMFAKKHAA YAWPFYKPVDVEALGLHDYC DIIKHPMDMSTIKSKLEARE YRDAQEFGADVRLMFSNCYK YNPPDHEVVAMARKLQDVFE MRFAKMLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	433
15N chemical shifts	103
1H chemical shifts	727

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Second bromodomain of Brd4	1

Entities:

Entity 1, Second bromodomain of Brd4 114 residues - Formula weight is not available

1

GLU

GLN

LEU

LYS

CYS

SER

GLY

ILE

LEU

2

LYS

GLU

MET

PHE

ALA

LYS

HIS

ALA

3

TYR

ALA

TRP

PRO

PHE

TYR

LYS

PRO

VAL

ASP

4

VAL

GLU

ALA

LEU

GLY

LEU

HIS

ASP

TYR

CYS

5

ASP

ILE

LYS

HIS

PRO

MET

ASP

MET

SER

6

THR

ILE

LYS

SER

LYS

LEU

GLU

ALA

ARG

GLU

7

TYR

ARG

ASP

ALA

GLN

GLU

PHE

GLY

ALA

ASP

8

VAL

ARG

LEU

MET

PHE

SER

ASN

CYS

TYR

LYS

9

TYR

ASN

PRO

ASP

HIS

GLU

VAL

ALA

10

MET

ALA

ARG

LYS

LEU

GLN

ASP

VAL

PHE

GLU

11

MET

ARG

PHE

ALA

LYS

MET

LEU

GLU

HIS

12

HIS

Samples:

sample: Bromodomain-containing protein 4, [U-13C; U-15N], 1.0 mM; phosphate buffer, none, 20 mM; NaCl, none, 50 mM; EDTA, none, 5 mM; D2O, none, 10 % v/v

sample_conditions_1: ionic strength: 70 mM; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample	isotropic	sample_conditions_1
3D 15N-separated NOESY	sample	isotropic	sample_conditions_1
2D NOESY	sample	isotropic	sample_conditions_1
2D TOCSY	sample	isotropic	sample_conditions_1

Software:

NMRPipe v2.3, F.Delaglio - processing

SPARKY v3, T.D.Goddard and D.G.Kneller - data analysis

CSI v1.0, David S.Wishart - data analysis

Molmol v2K.2, Koradi - data analysis

CNS v1.1, A Brunger, P Adams, M Clore, P Gros, M Nilges and R Read - refinement

NMR spectrometers:

Bruker DMX 600 MHz

PDB	2I8N 2LSP 2MJV 2OUO 2YEM 3JVL 3JVM 4KV4 4Z93
REF	XP_006524755 XP_006524756 XP_008763395 XP_011244845 XP_011382411