BMRB Entry 15052
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15052
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Citation: Ronga, Luisa; Palladino, Pasquale; Saviano, Gabriella; Tancredi, Teodorico; Benedetti, Ettore; Ragone, Raffaele; Rossi, Filomena. "Structural characterization of a neurotoxic threonine-rich peptide corresponding to the human prion protein alpha2-helical 180-195 segment, and comparison with full-length alpha2-helix-derived peptides" J. Pept. Sci. 14, 1096-1102 (2008).
PubMed: 18563793
Assembly members:
HPRP-173-195-D178N, polymer, 23 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
HPRP-173-195-D178N: NNFVHNCVNITIKQHTVTTT
TKG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 149 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mutated prion protein fragment | 1 |
Entities:
Entity 1, mutated prion protein fragment 23 residues - Formula weight is not available
| 1 | ASN | ASN | PHE | VAL | HIS | ASN | CYS | VAL | ASN | ILE | ||||
| 2 | THR | ILE | LYS | GLN | HIS | THR | VAL | THR | THR | THR | ||||
| 3 | THR | LYS | GLY |
Samples:
sample_1: HPRP-173-195-D178N1 – 2 mM; TRIFLUOROETHANOL-D2, [U-2H], 100%
sample_conditions_1: pH: 5.0; pressure: 1.0 atm; temperature: 300.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_1 | isotropic | sample_conditions_1 |
| COSY | sample_1 | isotropic | sample_conditions_1 |
| TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 15N-HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
DYANA, P.GUNTERT - refinement
NMRView, B Johnson, One Moon Scientific - structure solution
NMR spectrometers:
- Bruker AVANCE 600 MHz