BMRB Entry 15050

Title:
piscidin solution structure in presence of DPC micelles
Deposition date:
2006-11-22
Original release date:
2015-11-16
Authors:
Campagna, Sylvie; Saint, Nathalie; Molle, Gerard; Aumelas, Andre
Citation:

Citation: Campagna, Sylvie; Saint, Nathalie; Molle, Gerard; Aumelas, Andre. "Structure and mechanism of action of the antimicrobial peptide piscidin"  Biochemistry 46, 1771-1778 (2007).
PubMed: 17253775

Assembly members:

Assembly members:
piscidin-1, polymer, 23 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: striped bass   Taxonomy ID: 34816   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Morone saxatilis

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
piscidin-1: FFHHIFRGIVHVGKTIHRLV TGX

Data sets:
Data typeCount
1H chemical shifts160

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1piscidin-11

Entities:

Entity 1, piscidin-1 23 residues - Formula weight is not available

The C-terminus is amidated

1   PHEPHEHISHISILEPHEARGGLYILEVAL
2   HISVALGLYLYSTHRILEHISARGLEUVAL
3   THRGLYNH2

Samples:

sample_1: piscidin-1 1 ± 0.1 mM

sample_2: piscidin-1 1 ± 0.1 mM; DPC 39 mM

sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

DYANA v1.5, Guntert, Braun and Wuthrich - structure solution

xwinnmr, Bruker Biospin - chemical shift assignment, collection, processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19454 19455
PDB
GB AAL49496 AAL57319
SP Q8UUG0
AlphaFold Q8UUG0