BMRB Entry 15046

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15046
MolProbity Validation Chart

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Title:
1H, 13C, and 15N resonance assignments of subunit F of the A1AO ATP synthase from Methanosarcina mazei Go1
Deposition date:
2006-11-21
Original release date:
2007-06-26
Authors:
Gayen, Shovanlal; Vivekanandan, Subramanian; Biukovic, Goran; Gruber, Gerhard; Yoon, Ho Sup
Citation:

Citation: Gayen, Shovanlal; Vivekanandan, Subramanian; Biukovic, Goran; Gruber, Gerhard; Yoon, Ho Sup. "1H, 13C, and 15N resonance assignments of subunit F of the A(1)A (O) ATP synthase from Methanosarcina mazei Go1"  Biomol. NMR Assignments 1, 23-25 (2007).
PubMed: 19636817

Assembly members:

Assembly members:
F_subunit_A_type_ATP_synthase, polymer, 110 residues, 11944.677 Da.

Natural source:

Natural source:   Common Name: Methanosarcina mazei Go1   Taxonomy ID: 2209   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanosarcina mazei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet 9d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
F_subunit_A_type_ATP_synthase: MKHHHHHHPMELAVIGKSEF VTGFRLAGISKVYETPDIPA TESAVRSVLEDKSVGILVMH NDDIGNLPEVLRKNLNESVQ PTVVALGGSGSGSTSLREKI KQAVGVDLWK

Data sets:
Data typeCount
13C chemical shifts395
15N chemical shifts103
1H chemical shifts681

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit F1

Entities:

Entity 1, subunit F 110 residues - 11944.677 Da.

1   METLYSHISHISHISHISHISHISPROMET
2   GLULEUALAVALILEGLYLYSSERGLUPHE
3   VALTHRGLYPHEARGLEUALAGLYILESER
4   LYSVALTYRGLUTHRPROASPILEPROALA
5   THRGLUSERALAVALARGSERVALLEUGLU
6   ASPLYSSERVALGLYILELEUVALMETHIS
7   ASNASPASPILEGLYASNLEUPROGLUVAL
8   LEUARGLYSASNLEUASNGLUSERVALGLN
9   PROTHRVALVALALALEUGLYGLYSERGLY
10   SERGLYSERTHRSERLEUARGGLULYSILE
11   LYSGLNALAVALGLYVALASPLEUTRPLYS

Samples:

sample_1: F subunit A type ATP synthase, [U-13C; U-15N], 0.1 – 0.5 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.5; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB AAC06374 AAM30477 AGF96210 AKB39529 AKB60502
REF WP_011032731 WP_048048603 WP_054869506
SP Q60185
AlphaFold Q60185

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