BMRB Entry 15035

Name: 1H, and 15N Chemical Shift Assignments for the N-terminal domain of Myxococcus xantus CarA protein.
Published: 2007-02-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15035

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, and 15N Chemical Shift Assignments for the N-terminal domain of Myxococcus xantus CarA protein.

Deposition date:

2006-11-16

Original release date:

2007-02-08

Authors:

Jimenez, M. Angeles; Padmanabhan, S.; Gonzalez, Carlos; Perez-Marin, Mari; Elias-Arnanz, Montserrat; Murillo, Francisco; Rico, Manuel

Citation:

Citation: Navarro-Aviles, G.; Jimenez, M. Angeles; Perez-Marin, Mari; Gonzalez, Carlos; Rico, Manuel; Murillo, Francisco; Elias-Arnanz, Montserrat; Padmanabhan, S.. "Structural basis for operator and anti-repressor recognition by Myxococcus xanthus CarA repressor" Mol. Microbiol. 63, 980-994 (2007).

Assembly members:

Assembly members:
h6CarA(Nter), polymer, 98 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Myxococcus xanthus Taxonomy ID: 34 Superkingdom: Bacteria Kingdom: not available Genus/species: Myxococcus xanthus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
h6CarA(Nter): MGSSHHHHHHSSGLVPRGSH MTLRIRTIARMTGIREATLR AWERRYGFPRPLRSEGNNYR VYSREEVEAVRRVARLIQEE GLSVSEAIAQVKTEPPRE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	86
1H chemical shifts	504

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	h6CarA(Nter	1

Entities:

Entity 1, h6CarA(Nter 98 residues - Formula weight is not available

Residues -19 to 0 represent an N-terminal His tag used for purification.

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

THR

LEU

ARG

ILE

ARG

THR

ILE

ALA

ARG

4

MET

THR

GLY

ILE

ARG

GLU

ALA

THR

LEU

ARG

5

ALA

TRP

GLU

ARG

TYR

GLY

PHE

PRO

ARG

6

PRO

LEU

ARG

SER

GLU

GLY

ASN

TYR

ARG

7

VAL

TYR

SER

ARG

GLU

VAL

GLU

ALA

VAL

8

ARG

VAL

ALA

ARG

LEU

ILE

GLN

GLU

9

GLY

LEU

SER

VAL

SER

GLU

ALA

ILE

ALA

GLN

10

VAL

LYS

THR

GLU

PRO

ARG

GLU

Samples:

sample_1: h6CarA(Nter)0.5 – 1.0 mM; H2O 90%; D2O 10%; DSS0.1 – 0.5 mM; sodium chloride 100 mM; sodium phosphate 50 mM

sample_2: h6CarA(Nter)0.5 – 1.0 mM; D2O 100%; DSS0.1 – 0.5 mM; sodium chloride 100 mM; sodium phosphate 50 mM

sample_3: h6CarA(Nter), [U-100% 15N], 0.5 – 1.0 mM; H2O 90%; D2O 10%; DSS0.1 – 0.5 mM; sodium chloride 100 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 0.150 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_3	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_3	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_3	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_3	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_2	isotropic	sample_conditions_1

Software:

SPARKY, T Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

BMRB	15023
PDB	2JML
EMBL	CAA79964
GB	ABF89801
REF	WP_011551024
SP	Q50899
AlphaFold	Q50899