BMRB Entry 15033

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15033

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Title:
Dimeric solution structure of the cyclic octamer d(CCGTCCGT)
Deposition date:
2006-11-16
Original release date:
2007-10-16
Authors:
Escaja, N.; Gomez-Pinto, I.; Pedroso, E.; Gonzalez, C.
Citation:

Citation: Escaja, N.; Gomez-Pinto, I.; Pedroso, E.; Gonzalez, C.. "Four stranded DNA structures can be stabilized by two different types of minor groove G:C:G:C tetrads"  J. Am. Chem. Soc. 129, 2004-2014 (2007).
PubMed: 17260988

Assembly members:

Assembly members:
5'-D(P*CP*CP*GP*TP*CP*CP*GP*T)-3', polymer, 8 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
5'-D(P*CP*CP*GP*TP*CP*CP*GP*T)-3': CCGTCCGT

Data sets:
Data typeCount
13C chemical shifts20
1H chemical shifts43
31P chemical shifts4

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11
2subunit 21

Entities:

Entity 1, subunit 1 8 residues - Formula weight is not available

1   DCDCDGDTDCDCDGDT

Samples:

sample: 5'-D(P*CP*CP*GP*TP*CP*CP*GP*T)-3' 5 mM; sodium chloride 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 289 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsampleisotropicsample_conditions_1
2D DQF-COSYsampleisotropicsample_conditions_1

Software:

MARDIGRAS v1.5, T.L. James - iterative matrix relaxation

AMBER v7.0, Kollman et al - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 800 MHz