BMRB Entry 15025
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15025
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Duarte, Afonso; de Jong, Edwin; Wechselberger, Rainer; van Mierlo, Carlo; Hemminga, Marcus. "Segment TM7 from the cytoplasmic hemi-channel from VO-H+-V-ATPase includes a
flexible region that has a potential role in proton translocation." Biochim Biophys Acta. 1768, 2263-2270 (2007).
PubMed: 17573038
Assembly members:
sMTM7, polymer, 25 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
sMTM7: EFCLNCVSHTASYLRLWALS
LAHAQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 165 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | sMTM7 | 1 |
Entities:
Entity 1, sMTM7 25 residues - Formula weight is not available
This peptide mimicks the seventh transmembrane segmetne of subunit a from the yeast proton B-ATPase
| 1 | GLU | PHE | CYS | LEU | ASN | CYS | VAL | SER | HIS | THR | ||||
| 2 | ALA | SER | TYR | LEU | ARG | LEU | TRP | ALA | LEU | SER | ||||
| 3 | LEU | ALA | HIS | ALA | GLN |
Samples:
sample_1: sMTM7 2 mM; DMSO, [U-100% 2H], 100%
sample_conditions_1: temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, T Goddard - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker DRX 900 MHz