BMRB Entry 12041

Title:
1H, 13C and 15N resonance assignments of Q38FZ4, hypothetical protein from Trypanosoma brucei
Deposition date:
2020-01-15
Original release date:
2020-07-09
Authors:
Wang, Rui; Gao, Jie; Liao, Shanhui
Citation:

Citation: Wang, Rui; Zhang, Jiahai; Liao, Shanhui; Tu, Xiaoming. "1H, 13C and 15N resonance assignments of Q38FZ4, hypothetical protein from Trypanosoma brucei"  Biomol. NMR Assign. ., .-. (2020).
PubMed: 32488600

Assembly members:

Assembly members:
Q38FZ4, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Trypanosoma brucei   Taxonomy ID: 5691   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22(b)

Data sets:
Data typeCount
13C chemical shifts259
15N chemical shifts91
1H chemical shifts601

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Q38FZ41

Entities:

Entity 1, Q38FZ4 93 residues - Formula weight is not available

1   METALAPROALAVALILEGLUILEHISILE
2   PROLEUASPARGILEARGASNGLUGLUTYR
3   ALATHRASPASPLEULEULEUASNCYSLEU
4   SERLYSILEGLYASPTHRPROGLUGLUASP
5   GLYLEUPROLEUARGTHRTRPILELEUARG
6   GLUALAHISGLNALALEUILELYSSERPRO
7   LYSLEUARGTHRVALLEUVALLYSPROGLN
8   THRVALLYSASPLYSPROTHRHISPHEGLN
9   ILECYSPHEASPGLULEUGLUHISHISHIS
10   HISHISHIS

Samples:

sample_1: Q38FZ4, [U-99% 13C; U-99% 15N], 0.8 mM; NaH2PO4 25 mM; NaCl 150 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks