BMRB Entry 12018

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR12018

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Title:
Backbone resonance assignments of the YAP-binding domain of transcription factor TEAD4 of mouse
Deposition date:
2018-02-27
Original release date:
2018-07-17
Authors:
Li, Yan; Ng, Elizabeth Yihui; Kang, CongBao
Citation:

Citation: Li, Yan; Liu, Shuang; Ng, Elizabeth Yihui; Li, Rong; Poulsen, Anders; Hill, Jeffrey; Pobbati, Ajaybabu V.; Hung, Alvin W.; Hong, Wanjin; Keller, Thomas H.; Kang, CongBao. "Structural and ligand-binding analysis of the YAP-binding domain of transcription factor TEAD4"  Biochem. J. 475, 2043-2055 (2018).
PubMed: 29760238

Assembly members:

Assembly members:
YAP-binding_domain_of_transcription_factor_TEAD4, polymer, 239 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YAP-binding_domain_of_transcription_factor_TEAD4: MGSSHHHHHHSSGLVPRGSH MRSIASSKLWMLEFSAFLER QQDPDTYNKHLFVHISQSSP SYSDPYLETVDIRQIYDKFP EKKGGLKELFERGPSNAFFL VKFWADLNTNIDDEGSAFYG VSSQYESPENMIITCSTKVC SFGKQVVEKVETEYARYENG HYLYRIHRSPLCEYMINFIH KLKHLPEKYMMNSVLENFTI LQVVTNRDTQETLLCIAYVF EVSASEHGAQHHIYRLVKE

Data sets:
Data typeCount
13C chemical shifts277
15N chemical shifts141
1H chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YAP-binding domain of transcription factor TEAD41

Entities:

Entity 1, YAP-binding domain of transcription factor TEAD4 239 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METARGSERILEALASERSERLYSLEUTRP
4   METLEUGLUPHESERALAPHELEUGLUARG
5   GLNGLNASPPROASPTHRTYRASNLYSHIS
6   LEUPHEVALHISILESERGLNSERSERPRO
7   SERTYRSERASPPROTYRLEUGLUTHRVAL
8   ASPILEARGGLNILETYRASPLYSPHEPRO
9   GLULYSLYSGLYGLYLEULYSGLULEUPHE
10   GLUARGGLYPROSERASNALAPHEPHELEU
11   VALLYSPHETRPALAASPLEUASNTHRASN
12   ILEASPASPGLUGLYSERALAPHETYRGLY
13   VALSERSERGLNTYRGLUSERPROGLUASN
14   METILEILETHRCYSSERTHRLYSVALCYS
15   SERPHEGLYLYSGLNVALVALGLULYSVAL
16   GLUTHRGLUTYRALAARGTYRGLUASNGLY
17   HISTYRLEUTYRARGILEHISARGSERPRO
18   LEUCYSGLUTYRMETILEASNPHEILEHIS
19   LYSLEULYSHISLEUPROGLULYSTYRMET
20   METASNSERVALLEUGLUASNPHETHRILE
21   LEUGLNVALVALTHRASNARGASPTHRGLN
22   GLUTHRLEULEUCYSILEALATYRVALPHE
23   GLUVALSERALASERGLUHISGLYALAGLN
24   HISHISILETYRARGLEUVALLYSGLU

Samples:

sample_1: YAP-binding domain of transcription factor TEAD4, [U-13C; U-15N; U-2H], 0.8 mM; sodium phosphate 20 mM; sodium chloride 150 mM; EDTA 0.5 mM; DTT 1 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 170 mM; pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific, Keller and Wuthrich - chemical shift assignment, processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks