BMRB Entry 12002

Name: Backbone resonance assignments of the second LysM domain from Volvox carteri chitinase
Published: 2017-03-07

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR12002

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignments of the second LysM domain from Volvox carteri chitinase

Deposition date:

2016-06-13

Original release date:

2017-03-07

Authors:

Kitaoku, Yoshihito; Fukamizo, Tamo

Citation:

Citation: Kitaoku, Yoshihito; Fukamizo, Tamo; Numata, Tomoyuki; Ohnuma, Takayuki. "Chitin oligosaccharide binding to the lysin motif of a novel type of chitinase from the multicellular green alga, Volvox carteri" Plant Mol. Biol. 93, 97-108 (2017).
PubMed: 27807643

Assembly members:

Assembly members:
VcChi_LysM, polymer, 49 residues, Formula weight is not available

Natural source:

Natural source: Common Name: green algae Taxonomy ID: 3068 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Volvox Volvox carteri

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETBlue-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VcChi_LysM: MGCTYTIQPGDTFWAIAQRR GTTVDVIQSLNPGVNPARLQ VGQVINVPC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	142
15N chemical shifts	44
1H chemical shifts	44

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	VcChi LysM	1

Entities:

Entity 1, VcChi LysM 49 residues - Formula weight is not available

1

MET

GLY

CYS

THR

TYR

THR

ILE

GLN

PRO

GLY

2

ASP

THR

PHE

TRP

ALA

ILE

ALA

GLN

ARG

3

GLY

THR

VAL

ASP

VAL

ILE

GLN

SER

LEU

4

ASN

PRO

GLY

VAL

ASN

PRO

ALA

ARG

LEU

GLN

5

VAL

GLY

GLN

VAL

ILE

ASN

VAL

PRO

CYS

Samples:

sample_1: VcChi LysM, [U-100% 13C; U-100% 15N], 194 uM; sodium acetate 20 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 20 mM; pH: 5.0; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks