BMRB Entry 12002

Title:
Backbone resonance assignments of the second LysM domain from Volvox carteri chitinase
Deposition date:
2016-06-13
Original release date:
2017-03-07
Authors:
Kitaoku, Yoshihito; Fukamizo, Tamo
Citation:

Citation: Kitaoku, Yoshihito; Fukamizo, Tamo; Numata, Tomoyuki; Ohnuma, Takayuki. "Chitin oligosaccharide binding to the lysin motif of a novel type of chitinase from the multicellular green alga, Volvox carteri"  Plant Mol. Biol. 93, 97-108 (2017).
PubMed: 27807643

Assembly members:

Assembly members:
VcChi_LysM, polymer, 49 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: green algae   Taxonomy ID: 3068   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Volvox Volvox carteri

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETBlue-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VcChi_LysM: MGCTYTIQPGDTFWAIAQRR GTTVDVIQSLNPGVNPARLQ VGQVINVPC

Data sets:
Data typeCount
13C chemical shifts142
15N chemical shifts44
1H chemical shifts44

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VcChi LysM1

Entities:

Entity 1, VcChi LysM 49 residues - Formula weight is not available

1   METGLYCYSTHRTYRTHRILEGLNPROGLY
2   ASPTHRPHETRPALAILEALAGLNARGARG
3   GLYTHRTHRVALASPVALILEGLNSERLEU
4   ASNPROGLYVALASNPROALAARGLEUGLN
5   VALGLYGLNVALILEASNVALPROCYS

Samples:

sample_1: VcChi LysM, [U-100% 13C; U-100% 15N], 194 uM; sodium acetate 20 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 20 mM; pH: 5.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks