BMRB Entry 11599

Name: NMR structure of eIF1
Published: 2016-10-20

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PDB ID: 2rvh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11599
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of eIF1

Deposition date:

2015-10-16

Original release date:

2016-10-20

Authors:

NAGATA, Takashi; OBAYASHI, Eiji; ASANO, Katsura

Citation:

Citation: NAGATA, Takashi; OBAYASHI, Eiji. "NMR structure of eIF1" .

Assembly members:

Assembly members:
eIF1, polymer, 108 residues, 12330.247 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
eIF1: MSIENLKSFDPFADTGDDET ATSNYIHIRIQQRNGRKTLT TVQGVPEEYDLKRILKVLKK DFACNGNIVKDPEMGEIIQL QGDQRAKVCEFMISQLGLQK KNIKIHGF

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	473
15N chemical shifts	107
1H chemical shifts	738

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	eIF1	1

Entities:

Entity 1, eIF1 108 residues - 12330.247 Da.

1

MET

SER

ILE

GLU

ASN

LEU

LYS

SER

PHE

ASP

2

PRO

PHE

ALA

ASP

THR

GLY

ASP

GLU

THR

3

ALA

THR

SER

ASN

TYR

ILE

HIS

ILE

ARG

ILE

4

GLN

ARG

ASN

GLY

ARG

LYS

THR

LEU

THR

5

THR

VAL

GLN

GLY

VAL

PRO

GLU

TYR

ASP

6

LEU

LYS

ARG

ILE

LEU

LYS

VAL

LEU

LYS

7

ASP

PHE

ALA

CYS

ASN

GLY

ASN

ILE

VAL

LYS

8

ASP

PRO

GLU

MET

GLY

GLU

ILE

GLN

LEU

9

GLN

GLY

ASP

GLN

ARG

ALA

LYS

VAL

CYS

GLU

10

PHE

MET

ILE

SER

GLN

LEU

GLY

LEU

GLN

LYS

11

LYS

ASN

ILE

LYS

ILE

HIS

GLY

PHE

Samples:

sample_1: eIF1, [U-100% 13C; U-100% 15N], 0.4 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM; D2O, [U-2H], 5%; H2O 95%

sample_conditions_1: ionic strength: 170 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA v2.0.17, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, structure solution

Kujira v0.984, Naohira Kobayashi - chemical shift assignment, data analysis, peak picking

NMRView, Johnson - collection, data analysis, peak picking

NMRDraw, Delaglio - processing

NMRPipe, Delaglio - processing

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz