BMRB Entry 11581
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11581
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Mahajan, Mukesh; Bhattacharjya, Surajit. "NMR Structures and Localization of Potential Fusion Peptides and Pre-transmembrane Region of
SARS-CoV: Implications in Membrane Fusion" Biochim. Biophys. Acta ., .-..
Assembly members:
Pre_transmembrane_domain, polymer, 18 residues, 2378.794 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Pre_transmembrane_domain: LGKYEQYIKWPWYVWLGF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 16 |
| 1H chemical shifts | 104 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pre transmembrane domain | 1 |
Entities:
Entity 1, Pre transmembrane domain 18 residues - 2378.794 Da.
| 1 | LEU | GLY | LYS | TYR | GLU | GLN | TYR | ILE | LYS | TRP | ||||
| 2 | PRO | TRP | TYR | VAL | TRP | LEU | GLY | PHE |
Samples:
sample_1: Pre transmembrane domain 0.5 mM; DPC, [U-99% 2H], 125 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: pH: 4.7; temperature: 315 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | anisotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | anisotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | anisotropic | sample_conditions_1 |
Software:
SPARKY v3.113, Goddard - chemical shift assignment, data analysis
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - Structure calculation
NMR spectrometers:
- Bruker Avance 600 MHz