BMRB Entry 11575

Name: SOLUTION STRUCTURE OF THE SECOND PDZ DOMAIN OF HUMAN KIAA1526 PROTEIN
Published: 2014-10-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11575

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION STRUCTURE OF THE SECOND PDZ DOMAIN OF HUMAN KIAA1526 PROTEIN

Deposition date:

2014-09-08

Original release date:

2014-10-15

Authors:

Li, Hua; Tochio, Naoya; Koshiba, Seizo; Inoue, Makoto; Muto, Yutaka; Kigawa, Takanori; Yokoyama, Shigeyuki

Citation:

Citation: Li, Hua; Tochio, Naoya; Koshiba, Seizo; Inoue, Makoto; Muto, Yutaka; Kigawa, Takanori; Yokoyama, Shigeyuki. "SOLUTION STRUCTURE OF THE SECOND PDZ DOMAIN OF HUMAN KIAA1526 PROTEIN" .

Assembly members:

Assembly members:
PDZ_domain, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: E. coli - cell free Vector: P021030-45

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ_domain: GSSGSSGDRRSTLHLLQGGD EKKVNLVLGDGRSLGLTIRG GAEYGLGIYITGVDPGSEAE GSGLKVGDQILEVNGRSFLN ILHDEAVRLLKSSRHLILTV KDVGRLPHARTTVDETKWIA SSSGPSSG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	803
13C chemical shifts	516
15N chemical shifts	121

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PDZ domain	1

Entities:

Entity 1, PDZ domain 128 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASP

ARG

2

SER

THR

LEU

HIS

LEU

GLN

GLY

ASP

3

GLU

LYS

VAL

ASN

LEU

VAL

LEU

GLY

ASP

4

GLY

ARG

SER

LEU

GLY

LEU

THR

ILE

ARG

GLY

5

GLY

ALA

GLU

TYR

GLY

LEU

GLY

ILE

TYR

ILE

6

THR

GLY

VAL

ASP

PRO

GLY

SER

GLU

ALA

GLU

7

GLY

SER

GLY

LEU

LYS

VAL

GLY

ASP

GLN

ILE

8

LEU

GLU

VAL

ASN

GLY

ARG

SER

PHE

LEU

ASN

9

ILE

LEU

HIS

ASP

GLU

ALA

VAL

ARG

LEU

10

LYS

SER

ARG

HIS

LEU

ILE

LEU

THR

VAL

11

LYS

ASP

VAL

GLY

ARG

LEU

PRO

HIS

ALA

ARG

12

THR

VAL

ASP

GLU

THR

LYS

TRP

ILE

ALA

13

SER

GLY

PRO

SER

GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.6 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: temperature: 298 K; pH: 7.0; pressure: 1 atm; ionic strength: 120 mM

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, Kobayashi, N. - data analysis

NMR spectrometers:

Bruker Avance 800 MHz

PDB	1UF1
REF	XP_005581023 XP_005581024 XP_007966494 XP_009186476 XP_011853880