BMRB Entry 11574

Title:
SOLUTION STRUCTURE OF THE FIRST PDZ DOMAIN OF HUMAN KIAA1526 PROTEIN
Deposition date:
2014-09-04
Original release date:
2014-10-15
Authors:
Li, Hua; Tochio, Naoya; Koshiba, Seizo; Inoue, Makoto; Muto, Yutaka; Kigawa, Takanori; Yokoyama, Shigeyuki
Citation:

Citation: Li, Hua; Tochio, Naoya; Koshiba, Seizo; Inoue, Makoto; Muto, Yutaka; Kigawa, Takanori; Yokoyama, Shigeyuki. "SOLUTION STRUCTURE OF THE FIRST PDZ DOMAIN OF HUMAN KIAA1526 PROTEIN"  .

Assembly members:

Assembly members:
PDZ_domain, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: E. coli - cell free   Vector: P021030-45

Data sets:
Data typeCount
1H chemical shifts643
13C chemical shifts385
15N chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain1

Entities:

Entity 1, PDZ domain 101 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUVALARG
2   LEUVALSERLEUARGARGALALYSALAHIS
3   GLUGLYLEUGLYPHESERILEARGGLYGLY
4   SERGLUHISGLYVALGLYILETYRVALSER
5   LEUVALGLUPROGLYSERLEUALAGLULYS
6   GLUGLYLEUARGVALGLYASPGLNILELEU
7   ARGVALASNASPLYSSERLEUALAARGVAL
8   THRHISALAGLUALAVALLYSALALEULYS
9   GLYSERLYSLYSLEUVALLEUSERVALTYR
10   SERALAGLYARGILESERGLYPROSERSER
11   GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.6 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: temperature: 298 K; pH: 7.0; pressure: 1 atm; ionic strength: 120 mM

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, Kobayashi, N. - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB ELW70617 EPY80072
REF XP_012902069

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks