BMRB Entry 11574

Name: SOLUTION STRUCTURE OF THE FIRST PDZ DOMAIN OF HUMAN KIAA1526 PROTEIN
Published: 2014-10-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11574

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION STRUCTURE OF THE FIRST PDZ DOMAIN OF HUMAN KIAA1526 PROTEIN

Deposition date:

2014-09-04

Original release date:

2014-10-15

Authors:

Li, Hua; Tochio, Naoya; Koshiba, Seizo; Inoue, Makoto; Muto, Yutaka; Kigawa, Takanori; Yokoyama, Shigeyuki

Citation:

Citation: Li, Hua; Tochio, Naoya; Koshiba, Seizo; Inoue, Makoto; Muto, Yutaka; Kigawa, Takanori; Yokoyama, Shigeyuki. "SOLUTION STRUCTURE OF THE FIRST PDZ DOMAIN OF HUMAN KIAA1526 PROTEIN" .

Assembly members:

Assembly members:
PDZ_domain, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: E. coli - cell free Vector: P021030-45

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ_domain: GSSGSSGEVRLVSLRRAKAH EGLGFSIRGGSEHGVGIYVS LVEPGSLAEKEGLRVGDQIL RVNDKSLARVTHAEAVKALK GSKKLVLSVYSAGRISGPSS G

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	643
13C chemical shifts	385
15N chemical shifts	94

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PDZ domain	1

Entities:

Entity 1, PDZ domain 101 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

VAL

ARG

2

LEU

VAL

SER

LEU

ARG

ALA

LYS

ALA

HIS

3

GLU

GLY

LEU

GLY

PHE

SER

ILE

ARG

GLY

4

SER

GLU

HIS

GLY

VAL

GLY

ILE

TYR

VAL

SER

5

LEU

VAL

GLU

PRO

GLY

SER

LEU

ALA

GLU

LYS

6

GLU

GLY

LEU

ARG

VAL

GLY

ASP

GLN

ILE

LEU

7

ARG

VAL

ASN

ASP

LYS

SER

LEU

ALA

ARG

VAL

8

THR

HIS

ALA

GLU

ALA

VAL

LYS

ALA

LEU

LYS

9

GLY

SER

LYS

LEU

VAL

LEU

SER

VAL

TYR

10

SER

ALA

GLY

ARG

ILE

SER

GLY

PRO

SER

11

GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.6 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: temperature: 298 K; pH: 7.0; pressure: 1 atm; ionic strength: 120 mM

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, Kobayashi, N. - data analysis

NMR spectrometers:

Bruker Avance 800 MHz

PDB	1UEZ
GB	ELW70617 EPY80072
REF	XP_012902069