BMRB Entry 11555

Title:
Structure of the YAM domain of E. coli Transporter YajR   PubMed: 24952155
Deposition date:
2014-02-27
Original release date:
2014-06-30
Authors:
Liu, Xuehui; Feng, Wei
Citation:

Citation: Jiang, Daohua; Zhang, Xuejun. "Atomic Resolution Structure of the E. coli YajR Transporter YAM Domain"  Biochem. Biophys. Res. Commun. ., .-..

Assembly members:

Assembly members:
entity, polymer, 76 residues, 7876.034 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Experimental source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts285
15N chemical shifts68
1H chemical shifts458

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 76 residues - 7876.034 Da.

1   METGLYLYSGLUPROPROTYRVALSERSER
2   LEUARGILEGLUILEPROALAASPILEALA
3   ALAASNGLUALALEULYSVALARGLEULEU
4   GLUTHRGLUGLYVALLYSGLUVALLEUILE
5   ALAGLUGLUGLUHISSERALATYRVALLYS
6   ILEASPSERLYSVALTHRASNARGPHEGLU
7   VALGLUGLNALAILEARGGLNALALEUGLU
8   HISHISHISHISHISHIS

Samples:

sample_1: YAM 1.2 mM; H2O 90%; D2O 10%; sodium phosphate 50 mM; EDTA 1 mM; DTT 1 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 6.3; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

ARIA v2.3, Linge, O'Donoghue and Nilges - chemical shift assignment, structure solution

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAG75973 BAI23798 BAI29269 BAI34436 BAI53927
EMBL CAP74961 CAQ30896 CAQ90093 CAQ97299 CAR01770
GB AAN79016 AAZ87180 ABB60522 ABE05951 ABG68516
REF WP_001000941 WP_001000945 WP_001000947 WP_001000948 WP_001000951

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks