BMRB Entry 11542

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for yeast Tim50 PBD domain
Published: 2014-03-25

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11542

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for yeast Tim50 PBD domain

Deposition date:

2013-11-12

Original release date:

2014-03-25

Authors:

Rahman, Bytul; Anzai, Takahiro; Kawano, Shin; Endo, Toshiya

Citation:

Citation: Rahman, Bytul; Kawano, Shin; Esaki, Kaori; Anzai, Takahiro; Endo, Toshiya. "NMR analyses on the interactions of the yeast Tim50 C-terminal region with the presequence and Tim50 core domain." FEBS Lett. 588, 678-684 (2014).
PubMed: 24462684

Assembly members:

Assembly members:
Tim50_PBD, polymer, 72 residues, 8400 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tim50_PBD: MGSSHHHHHHSSGLVPRGSH MEEGQKNYLMFMKMIEEEKE KIRIQQEQMGGQTFTLKDYV EGNLPSPEEQMK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	160
15N chemical shifts	55
1H chemical shifts	55

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Tim50 PBD	1

Entities:

Entity 1, Tim50 PBD 72 residues - 8400 Da.

Residues 379-399 represent a non-native affinity His-tag.

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

GLU

GLY

GLN

LYS

ASN

TYR

LEU

MET

4

PHE

MET

LYS

MET

ILE

GLU

LYS

GLU

5

LYS

ILE

ARG

ILE

GLN

GLU

GLN

MET

GLY

6

GLY

GLN

THR

PHE

THR

LEU

LYS

ASP

TYR

VAL

7

GLU

GLY

ASN

LEU

PRO

SER

PRO

GLU

GLN

8

MET

LYS

Samples:

sample_1: Tim50 sPBD, [U-100% 13C; U-100% 15N], 100 uM; potassium phosphate 20 mM; potassium chloride 50 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 70 mM; pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCANNH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.114, Goddard - chemical shift assignment

TOPSPIN v1.3, Bruker Biospin - processing

NMR spectrometers:

Bruker Avance 600 MHz

DBJ	GAA26910
EMBL	CAY86898
GB	AAB68302 AHY78114 AJP42082 AJV91172 AJV91610
REF	NP_015262
SP	Q02776
TPG	DAA11368
AlphaFold	Q02776