BMRB Entry 11538
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11538
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Kushibiki, Takahiro; Kamiya, Masakatsu; Aizawa, Tomoyasu; Kumaki, Yasuhiro; Kikukawa, Takashi; Mizuguchi, Mineyuki; Demura, Makoto; Kawabata, Syun-ichiro; Kawano, Keiichi. "Interaction between tachyplesin I, an antimicrobial peptide derived from horseshoe crab, and lipopolysaccharide." Biochim. Biophys. Acta 1844, 527-534 (2014).
PubMed: 24389234
Assembly members:
Tachyplesin_I, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: Tachypleus tridentatus Taxonomy ID: 6853 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Tachypleus tridentatus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Tachyplesin_I: KWCFRVCYRGICYRRCRX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 121 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TPI | 1 |
Entities:
Entity 1, TPI 18 residues - Formula weight is not available
| 1 | LYS | TRP | CYS | PHE | ARG | VAL | CYS | TYR | ARG | GLY | ||||
| 2 | ILE | CYS | TYR | ARG | ARG | CYS | ARG | NH2 |
Samples:
sample_1: Tachyplesin I 750 uM; Lipopolysaccharide 50 uM; H2O 90%; D2O 10%
sample_conditions_1: pH: 3.8; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker DMX 500 MHz
- JEOL ECA 600 MHz