BMRB Entry 11527

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for PriB
Published: 2017-03-13

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11527

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for PriB

Deposition date:

2013-06-08

Original release date:

2017-03-13

Authors:

Abe, Yoshito; Takenawa, Taichi; Shioi, Seijiro; Fujiyama, Saki; Aramaki, Takahiko; Katayama, Tsutomu; Ueda, Tadashi

Citation:

Citation: Fujiyama, Saki; Abe, Yoshito; Takenawa, Taichi; Aramaki, Takahiko; Shioi, Seijiro; Katayama, Tsutomu; Ueda, Tadashi. "Involvement of histidine in complex formation of PriB and single-stranded DNA" Biochim. Biophys. Acta 1844, 299-307 (2014).
PubMed: 24200676

Assembly members:

Assembly members:
PriB, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PriB: MTNRLVLSGTVCRAPLRKVS PSGIPHCQFVLEHRSVQEEA GFHRQAWCQMPVIVSGHENQ AITHSITVGSRITVQGFISC HKAKNGLSKMVLHAEQIELI DSGD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	282
15N chemical shifts	93
1H chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PriB homodimer, subunit 1	1
2	PriB homodimer, subunit 2	1

Entities:

Entity 1, PriB homodimer, subunit 1 104 residues - Formula weight is not available

1

MET

THR

ASN

ARG

LEU

VAL

LEU

SER

GLY

THR

2

VAL

CYS

ARG

ALA

PRO

LEU

ARG

LYS

VAL

SER

3

PRO

SER

GLY

ILE

PRO

HIS

CYS

GLN

PHE

VAL

4

LEU

GLU

HIS

ARG

SER

VAL

GLN

GLU

ALA

5

GLY

PHE

HIS

ARG

GLN

ALA

TRP

CYS

GLN

MET

6

PRO

VAL

ILE

VAL

SER

GLY

HIS

GLU

ASN

GLN

7

ALA

ILE

THR

HIS

SER

ILE

THR

VAL

GLY

SER

8

ARG

ILE

THR

VAL

GLN

GLY

PHE

ILE

SER

CYS

9

HIS

LYS

ALA

LYS

ASN

GLY

LEU

SER

LYS

MET

10

VAL

LEU

HIS

ALA

GLU

GLN

ILE

GLU

LEU

ILE

11

ASP

SER

GLY

ASP

Samples:

sample_1: PriB, [U-98% 13C; U-98% 15N], 0.5 – 0.8 mM; HEPES 20 mM; ammonium sulfate 200 mM; D2O 10%; H2O 90%; DTT 5 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Olivia, hokkaido university - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz