BMRB Entry 11516

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11516

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Title:
THE SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF HUMAN ACTIVATOR OF 90 KDA HEAT SHOCK PROTEIN ATPASE HOMOLOG 1
Deposition date:
2012-11-19
Original release date:
2012-12-10
Authors:
Tochio, Naoya; Koshiba, Seizo; Inoue, Makoto; Kigawa, Takanori; Yokoyama, Shigeyuki
Citation:

Citation: Tochio, Naoya; Koshiba, Seizo; Kigawa, Takanori; Yokoyama, Shigeyuki. "THE SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF HUMAN ACTIVATOR OF 90 KDA HEAT SHOCK PROTEIN ATPASE HOMOLOG 1"  .

Assembly members:

Assembly members:
protein, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: E. coli - cell free   Vector: P040524-05

Entity Sequences (FASTA):

Entity Sequences (FASTA):
protein: GSSGSSGIPTCKITLKETFL TSPEELYRVFTTQELVQAFT HAPATLEADRGGKFHMVDGN VSGEFTDLVPEKHIVMKWRF KSWPEGHFATITLTFIDKNG ETELCMEGRGIPAPEEERTR QGWQRYYFEGIKQTFGYGAS GPSSG

Data sets:
Data typeCount
13C chemical shifts639
15N chemical shifts138
1H chemical shifts957

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AHA1 C-terminal domain1

Entities:

Entity 1, AHA1 C-terminal domain 145 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYILEPROTHR
2   CYSLYSILETHRLEULYSGLUTHRPHELEU
3   THRSERPROGLUGLULEUTYRARGVALPHE
4   THRTHRGLNGLULEUVALGLNALAPHETHR
5   HISALAPROALATHRLEUGLUALAASPARG
6   GLYGLYLYSPHEHISMETVALASPGLYASN
7   VALSERGLYGLUPHETHRASPLEUVALPRO
8   GLULYSHISILEVALMETLYSTRPARGPHE
9   LYSSERTRPPROGLUGLYHISPHEALATHR
10   ILETHRLEUTHRPHEILEASPLYSASNGLY
11   GLUTHRGLULEUCYSMETGLUGLYARGGLY
12   ILEPROALAPROGLUGLUGLUARGTHRARG
13   GLNGLYTRPGLNARGTYRTYRPHEGLUGLY
14   ILELYSGLNTHRPHEGLYTYRGLYALASER
15   GLYPROSERSERGLY

Samples:

sample_1: protein, [U-13C; U-15N], 1.3 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, N. Kobayashi - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

PDB
DBJ BAC36160 BAE38913 BAG36559 BAG62431
EMBL CAB45684 CAH92050
GB AAD09623 AAF29000 AAF80755 AAH00321 AAH07398
REF NP_001108506 NP_001127499 NP_001253813 NP_036243 NP_666148
SP O95433 Q8BK64
AlphaFold O95433 Q8BK64

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks