BMRB Entry 11515

Name: goat alpha-lactalbumin at pH 2.0
Published: 2017-03-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11515

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

goat alpha-lactalbumin at pH 2.0

Deposition date:

2012-10-30

Original release date:

2017-03-13

Authors:

Nakamura, Takashi; Aizawa, Tomoyasu; Demura, Makoto; Kawano, Keiichi; Makabe, Koki; Kuwajima, Kunihiro

Citation:

Citation: Nakamura, Takashi; Aizawa, Tomoyasu; Kariya, Ryusho; Okada, Seiji; Demura, Makoto; Kawano, Keiichi; Makabe, Koki; Kuwajima, Kunihiro. "Molecular mechanisms of the cytotoxicity of human alpha-lactalbumin made lethal to tumor cells (HAMLET) and other protein-oleic acid complexes" J. Biol. Chem. 288, 14408-14416 (2013).
PubMed: 23580643

Assembly members:

Assembly members:
goat_alpha-lactalbumin, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Capra Taxonomy ID: 9922 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: not available Capra

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSCREEN-LA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
goat_alpha-lactalbumin: EQLTKCEVFQKLKDLKDYGG VSLPEWVCTAFHTSGYDTQA IVQNNDSTEYGLFQINNKIW CKDDQNPHSRNICNISCDKF LDDDLTDDIVCAKKILDKVG INYWLAHKALCSEKLDQWLC EKL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	40
1H chemical shifts	40

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	goat alpha-lactalbumin	1

Entities:

Entity 1, goat alpha-lactalbumin 123 residues - Formula weight is not available

1

GLU

GLN

LEU

THR

LYS

CYS

GLU

VAL

PHE

GLN

2

LYS

LEU

LYS

ASP

LEU

LYS

ASP

TYR

GLY

3

VAL

SER

LEU

PRO

GLU

TRP

VAL

CYS

THR

ALA

4

PHE

HIS

THR

SER

GLY

TYR

ASP

THR

GLN

ALA

5

ILE

VAL

GLN

ASN

ASP

SER

THR

GLU

TYR

6

GLY

LEU

PHE

GLN

ILE

ASN

LYS

ILE

TRP

7

CYS

LYS

ASP

GLN

ASN

PRO

HIS

SER

ARG

8

ASN

ILE

CYS

ASN

ILE

SER

CYS

ASP

LYS

PHE

9

LEU

ASP

LEU

THR

ASP

ILE

VAL

10

CYS

ALA

LYS

ILE

LEU

ASP

LYS

VAL

GLY

11

ILE

ASN

TYR

TRP

LEU

ALA

HIS

LYS

ALA

LEU

12

CYS

SER

GLU

LYS

LEU

ASP

GLN

TRP

LEU

CYS

13

GLU

LYS

LEU

Samples:

sample_1: goat alpha-lactalbumin, [U-95% 13C; U-95% 15N], 300 uM; D2O 10%; H2O 90%

sample_conditions_1: pH: 2.0; pressure: 1 atm; temperature: 323.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMR spectrometers:

JEOL ECA 920 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks