BMRB Entry 11511

Name: acidic intrinsically disordered region of SSRP1
Published: 2018-12-18

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11511

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

acidic intrinsically disordered region of SSRP1

Deposition date:

2012-09-21

Original release date:

2018-12-18

Authors:

Hashimoto, Manami; Kodera, Noriyuki; Tsunaka, Yasuo; Ando, Toshio; Morikawa, Kosuke; Tate, Shin-ichi

Citation:

Citation: Hashimoto, Manami; Kodera, Noriyuki; Tsunaka, Yasuo; Oda, Masayuki; Tanimoto, Mitsuru; Ando, Toshio; Morikawa, Kosuke; Tate, Shin-ichi. "Phosphorylation-coupled intramolecular dynamics of unstructured regions in chromatin remodeler FACT." Biophys. J. 104, 2222-2234 (2013).
PubMed: 23708362

Assembly members:

Assembly members:
acidic_intrinsically_disordered_region_of_SSRP1, polymer, 89 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
acidic_intrinsically_disordered_region_of_SSRP1: GSHMGYKDVDFGDSDNENEP DAYLARLKAEAREKEEDDDD GDSDEESTDEDFKPNENESD VAEEYDSNVESDSDDDSDAS GGGGDSDGA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	225
15N chemical shifts	79
1H chemical shifts	79

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	polypeptide	1

Entities:

Entity 1, polypeptide 89 residues - Formula weight is not available

1

GLY

SER

HIS

MET

GLY

TYR

LYS

ASP

VAL

ASP

2

PHE

GLY

ASP

SER

ASP

ASN

GLU

ASN

GLU

PRO

3

ASP

ALA

TYR

LEU

ALA

ARG

LEU

LYS

ALA

GLU

4

ALA

ARG

GLU

LYS

GLU

ASP

5

GLY

ASP

SER

ASP

GLU

SER

THR

ASP

GLU

6

ASP

PHE

LYS

PRO

ASN

GLU

ASN

GLU

SER

ASP

7

VAL

ALA

GLU

TYR

ASP

SER

ASN

VAL

GLU

8

SER

ASP

SER

ASP

SER

ASP

ALA

SER

9

GLY

ASP

SER

ASP

GLY

ALA

Samples:

sample_1: acidic intrinsically disordered region of SSRP1, [U-13C; U-15N], 1 mM; sodium phosphate 50 mM; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 50 mM; pH: 6.4; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCANNH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks