BMRB Entry 11499

Name: Solution structure of RNF146 WWE domain
Published: 2018-12-18

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11499

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of RNF146 WWE domain

Deposition date:

2012-05-08

Original release date:

2018-12-18

Authors:

He, Fahu; Muto, Yutaka; Inoue, Makoto; Kigawa, Takanori; Mikako, Mikako; Terada, Takaho; Yokoyama, Shigeyuki

Citation:

Citation: He, Fahu; Tsuda, Kengo; Takahashi, Mari; Kuwasako, Kanako; Terada, Takano; Shirouzu, Mikako; Watanabe, Satoru; Kigawa, Takanori; Kobayashi, Naohiro; Guntert, Peter; Yokoyama, Shigeyuki; Muto, Yutaka. "Structural insight into the interaction of ADP-ribose with the PARP WWE domains." FEBS Lett. 586, 3858-3864 (2012).
PubMed: 23010590

Assembly members:

Assembly members:
RNF146, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: E. coli - cell free Vector: P-000001

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNF146: PSSGSSGFLDKPTLLSPEEL KAASRGNGEYAWYYEGRNGW WQYDERTSRELEDAFSKGKK NTEMLIAGFLYVADLENMVQ YRRNEHGRRRKIKRDIIDIP KKGVSGPSSG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	467
15N chemical shifts	112
1H chemical shifts	730

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WWE	1

Entities:

Entity 1, WWE 110 residues - Formula weight is not available

1	PRO	SER	SER	GLY	SER	SER	GLY	PHE	LEU	ASP
2	LYS	PRO	THR	LEU	LEU	SER	PRO	GLU	GLU	LEU
3	LYS	ALA	ALA	SER	ARG	GLY	ASN	GLY	GLU	TYR
4	ALA	TRP	TYR	TYR	GLU	GLY	ARG	ASN	GLY	TRP
5	TRP	GLN	TYR	ASP	GLU	ARG	THR	SER	ARG	GLU
6	LEU	GLU	ASP	ALA	PHE	SER	LYS	GLY	LYS	LYS
7	ASN	THR	GLU	MET	LEU	ILE	ALA	GLY	PHE	LEU
8	TYR	VAL	ALA	ASP	LEU	GLU	ASN	MET	VAL	GLN
9	TYR	ARG	ARG	ASN	GLU	HIS	GLY	ARG	ARG	ARG
10	LYS	ILE	LYS	ARG	ASP	ILE	ILE	ASP	ILE	PRO
11	LYS	LYS	GLY	VAL	SER	GLY	PRO	SER	SER	GLY

Samples:

sample_1: WWE, [U-99% 13C; U-99% 15N], 1.0 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - chemical shift assignment

Kujira, N. Kobayashi - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	PRO	SER	SER	GLY	SER	SER	GLY	PHE	LEU	ASP
2	LYS	PRO	THR	LEU	LEU	SER	PRO	GLU	GLU	LEU
3	LYS	ALA	ALA	SER	ARG	GLY	ASN	GLY	GLU	TYR
4	ALA	TRP	TYR	TYR	GLU	GLY	ARG	ASN	GLY	TRP
5	TRP	GLN	TYR	ASP	GLU	ARG	THR	SER	ARG	GLU
6	LEU	GLU	ASP	ALA	PHE	SER	LYS	GLY	LYS	LYS
7	ASN	THR	GLU	MET	LEU	ILE	ALA	GLY	PHE	LEU
8	TYR	VAL	ALA	ASP	LEU	GLU	ASN	MET	VAL	GLN
9	TYR	ARG	ARG	ASN	GLU	HIS	GLY	ARG	ARG	ARG
10	LYS	ILE	LYS	ARG	ASP	ILE	ILE	ASP	ILE	PRO
11	LYS	LYS	GLY	VAL	SER	GLY	PRO	SER	SER	GLY

1	PRO	SER	SER	GLY	SER	SER	GLY	PHE	LEU	ASP
2	LYS	PRO	THR	LEU	LEU	SER	PRO	GLU	GLU	LEU
3	LYS	ALA	ALA	SER	ARG	GLY	ASN	GLY	GLU	TYR
4	ALA	TRP	TYR	TYR	GLU	GLY	ARG	ASN	GLY	TRP
5	TRP	GLN	TYR	ASP	GLU	ARG	THR	SER	ARG	GLU
6	LEU	GLU	ASP	ALA	PHE	SER	LYS	GLY	LYS	LYS
7	ASN	THR	GLU	MET	LEU	ILE	ALA	GLY	PHE	LEU
8	TYR	VAL	ALA	ASP	LEU	GLU	ASN	MET	VAL	GLN
9	TYR	ARG	ARG	ASN	GLU	HIS	GLY	ARG	ARG	ARG
10	LYS	ILE	LYS	ARG	ASP	ILE	ILE	ASP	ILE	PRO
11	LYS	LYS	GLY	VAL	SER	GLY	PRO	SER	SER	GLY

1	PRO	SER	SER	GLY	SER	SER	GLY	PHE	LEU	ASP
2	LYS	PRO	THR	LEU	LEU	SER	PRO	GLU	GLU	LEU
3	LYS	ALA	ALA	SER	ARG	GLY	ASN	GLY	GLU	TYR
4	ALA	TRP	TYR	TYR	GLU	GLY	ARG	ASN	GLY	TRP
5	TRP	GLN	TYR	ASP	GLU	ARG	THR	SER	ARG	GLU
6	LEU	GLU	ASP	ALA	PHE	SER	LYS	GLY	LYS	LYS
7	ASN	THR	GLU	MET	LEU	ILE	ALA	GLY	PHE	LEU
8	TYR	VAL	ALA	ASP	LEU	GLU	ASN	MET	VAL	GLN
9	TYR	ARG	ARG	ASN	GLU	HIS	GLY	ARG	ARG	ARG
10	LYS	ILE	LYS	ARG	ASP	ILE	ILE	ASP	ILE	PRO
11	LYS	LYS	GLY	VAL	SER	GLY	PRO	SER	SER	GLY