BMRB Entry 11498

Name: Backbone resonance assignment for the unique and SH3 domains of mouse lymphocyte specific kinase
Published: 2019-09-05

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11498

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignment for the unique and SH3 domains of mouse lymphocyte specific kinase

Deposition date:

2012-05-04

Original release date:

2019-09-05

Authors:

Li, Hua; Xu, Chenqi

Citation:

Citation: Li, Lunyi; Guo, Xingdong; Shi, Xiaoshan; Li, Changting; Wu, Wei; Yan, Chengsong; Wang, Haopeng; Li, Hua; Xu, Chenqi. "Ionic CD3-Lck interaction regulates the initiation of T-cell receptor signaling" Proc. Natl. Acad. Sci. U.S.A. 114, E5891-E5899 (2017).
PubMed: 28659468

Assembly members:

Assembly members:
Unique_and_SH3_domains_of_mouse_Lck, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: N/A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Unique_and_SH3_domains_of_mouse_Lck: GSSNPEDDWMENIDVCENCH YPIVPLDSKISLPIRNGSEV RDPLVTYEGSLPPASPLQDN LVIALHSYEPSHDGDLGFEK GEQLRILEQSGEWWKAQSLT TGQEGFIPFNFVAKANS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	335
15N chemical shifts	102
1H chemical shifts	102

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Unique and SH3 domains of mouse Lck	1

Entities:

Entity 1, Unique and SH3 domains of mouse Lck 117 residues - Formula weight is not available

1

GLY

SER

ASN

PRO

GLU

ASP

TRP

MET

2

GLU

ASN

ILE

ASP

VAL

CYS

GLU

ASN

CYS

HIS

3

TYR

PRO

ILE

VAL

PRO

LEU

ASP

SER

LYS

ILE

4

SER

LEU

PRO

ILE

ARG

ASN

GLY

SER

GLU

VAL

5

ARG

ASP

PRO

LEU

VAL

THR

TYR

GLU

GLY

SER

6

LEU

PRO

ALA

SER

PRO

LEU

GLN

ASP

ASN

7

LEU

VAL

ILE

ALA

LEU

HIS

SER

TYR

GLU

PRO

8

SER

HIS

ASP

GLY

ASP

LEU

GLY

PHE

GLU

LYS

9

GLY

GLU

GLN

LEU

ARG

ILE

LEU

GLU

GLN

SER

10

GLY

GLU

TRP

LYS

ALA

GLN

SER

LEU

THR

11

THR

GLY

GLN

GLU

GLY

PHE

ILE

PRO

PHE

ASN

12

PHE

VAL

ALA

LYS

ALA

ASN

SER

Samples:

sample_1: Unique and SH3 domains of mouse Lck, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%; sodium phosphate 18 mM

sample_conditions_1: ionic strength: 18 mM; pH: 7.0; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

Kujira, Naohiro Kobayashi - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks