BMRB Entry 11498

Title:
Backbone resonance assignment for the unique and SH3 domains of mouse lymphocyte specific kinase
Deposition date:
2012-05-04
Original release date:
2019-09-05
Authors:
Li, Hua; Xu, Chenqi
Citation:

Citation: Li, Lunyi; Guo, Xingdong; Shi, Xiaoshan; Li, Changting; Wu, Wei; Yan, Chengsong; Wang, Haopeng; Li, Hua; Xu, Chenqi. "Ionic CD3-Lck interaction regulates the initiation of T-cell receptor signaling"  Proc. Natl. Acad. Sci. U.S.A. 114, E5891-E5899 (2017).
PubMed: 28659468

Assembly members:

Assembly members:
Unique_and_SH3_domains_of_mouse_Lck, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: N/A

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts335
15N chemical shifts102
1H chemical shifts102

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Unique and SH3 domains of mouse Lck1

Entities:

Entity 1, Unique and SH3 domains of mouse Lck 117 residues - Formula weight is not available

1   GLYSERSERASNPROGLUASPASPTRPMET
2   GLUASNILEASPVALCYSGLUASNCYSHIS
3   TYRPROILEVALPROLEUASPSERLYSILE
4   SERLEUPROILEARGASNGLYSERGLUVAL
5   ARGASPPROLEUVALTHRTYRGLUGLYSER
6   LEUPROPROALASERPROLEUGLNASPASN
7   LEUVALILEALALEUHISSERTYRGLUPRO
8   SERHISASPGLYASPLEUGLYPHEGLULYS
9   GLYGLUGLNLEUARGILELEUGLUGLNSER
10   GLYGLUTRPTRPLYSALAGLNSERLEUTHR
11   THRGLYGLNGLUGLYPHEILEPROPHEASN
12   PHEVALALALYSALAASNSER

Samples:

sample_1: Unique and SH3 domains of mouse Lck, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%; sodium phosphate 18 mM

sample_conditions_1: ionic strength: 18 mM; pH: 7.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

Kujira, Naohiro Kobayashi - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks