BMRB Entry 11493

Name: NMR chemical shift of Streptomyces sp. N174 chitosanase
Published: 2012-05-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11493

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR chemical shift of Streptomyces sp. N174 chitosanase

Deposition date:

2012-04-09

Original release date:

2012-05-02

Authors:

Shinya, Shoko; Brzezinski, Ryszard; Fukamizo, Tamo

Citation:

Citation: Shinya, Shoko; Brzezinski, Ryszard; Fukamizo, Tamo. "Chitosan oligosaccharide binding to GH-46 chitosanase from Streptomyces sp. N174 as determined by NMR spectroscopy" FEBS J. ., .-..

Assembly members:

Assembly members:
CsnN174, polymer, 238 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Streptomyces sp. Taxonomy ID: 1931 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces sp.

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Streptomyces lividans Vector: pFD666

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CsnN174: AGAGLDDPHKKEIAMELVSS AENSSLDWKAQYKYIEDIGD GRGYTGGIIGFCSGTGDMLE LVQHYTDLEPGNILAKYLPA LKKVNGSASHSGLGTPFTKD WATAAKDTVFQQAQNDERDR VYFDPAVSQAKADGLRALGQ FAYYDAIVMHGPGNDPTSFG GIRKTAMKKARTPAQGGDET TYLNGFLDARKAAMLTEAAH DDTSRVDTEQRVFLKAGNLD LNPPLKWKTYGDPYVINS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	399
15N chemical shifts	162
1H chemical shifts	162

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CsnN174	1

Entities:

Entity 1, CsnN174 238 residues - Formula weight is not available

1

ALA

GLY

ALA

GLY

LEU

ASP

PRO

HIS

LYS

2

LYS

GLU

ILE

ALA

MET

GLU

LEU

VAL

SER

3

ALA

GLU

ASN

SER

LEU

ASP

TRP

LYS

ALA

4

GLN

TYR

LYS

TYR

ILE

GLU

ASP

ILE

GLY

ASP

5

GLY

ARG

GLY

TYR

THR

GLY

ILE

GLY

6

PHE

CYS

SER

GLY

THR

GLY

ASP

MET

LEU

GLU

7

LEU

VAL

GLN

HIS

TYR

THR

ASP

LEU

GLU

PRO

8

GLY

ASN

ILE

LEU

ALA

LYS

TYR

LEU

PRO

ALA

9

LEU

LYS

VAL

ASN

GLY

SER

ALA

SER

HIS

10

SER

GLY

LEU

GLY

THR

PRO

PHE

THR

LYS

ASP

11

TRP

ALA

THR

ALA

LYS

ASP

THR

VAL

PHE

12

GLN

ALA

GLN

ASN

ASP

GLU

ARG

ASP

ARG

13

VAL

TYR

PHE

ASP

PRO

ALA

VAL

SER

GLN

ALA

14

LYS

ALA

ASP

GLY

LEU

ARG

ALA

LEU

GLY

GLN

15

PHE

ALA

TYR

ASP

ALA

ILE

VAL

MET

HIS

16

GLY

PRO

GLY

ASN

ASP

PRO

THR

SER

PHE

GLY

17

GLY

ILE

ARG

LYS

THR

ALA

MET

LYS

ALA

18

ARG

THR

PRO

ALA

GLN

GLY

ASP

GLU

THR

19

THR

TYR

LEU

ASN

GLY

PHE

LEU

ASP

ALA

ARG

20

LYS

ALA

MET

LEU

THR

GLU

ALA

HIS

21

ASP

THR

SER

ARG

VAL

ASP

THR

GLU

GLN

22

ARG

VAL

PHE

LEU

LYS

ALA

GLY

ASN

LEU

ASP

23

LEU

ASN

PRO

LEU

LYS

TRP

LYS

THR

TYR

24

GLY

ASP

PRO

TYR

VAL

ILE

ASN

SER

Samples:

sample_1: N174Csn, [U-13C; U-15N], 0.1 mM; sodium acetate 50 mM; D2O, [U-99% 2H], 5%; H2O 95%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz

BMRB	11494
PDB	1CHK
GB	AAA19865
SP	P33665
AlphaFold	P33665