BMRB Entry 11491

Name: Solution structure and siRNA-mediated knockdown analysis of the mitochondrial disease-related protein C12orf65 (ICT2)
Published: 2012-08-06

Click here to enlarge.

PDB ID: 2rsm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11491
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure and siRNA-mediated knockdown analysis of the mitochondrial disease-related protein C12orf65 (ICT2)

Deposition date:

2012-03-28

Original release date:

2012-08-06

Authors:

Enomoto, Mayu; Tochio, Naoya; Tomizawa, Tadashi; Koshiba, Seizo; Guntert, Peter; Kigawa, Takanori; Yokoyama, Shigeyuki; Nameki, Nobukazu

Citation:

Citation: Kogure, H.; Hikawa, Y.; Hagihara, M.; Tochio, N.; Koshiba, S.; Inoue, Y.; Guntert, P.; Kigawa, T.; Yokoyama, S.; Nameki, N.. "Solution structure and siRNA-mediated knockdown analysis of the mitochondrial disease-related protein C12orf65" Proteins ., .-. (2012).
PubMed: 22821833

Assembly members:

Assembly members:
entity, polymer, 115 residues, 12305.086 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P070213-30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GSSGSSGKWGLRLQEKPALL FPGMAASTVQVAGRKDYPAL LPLNESELEEQFVKGHGPGG QATNKTSNCVVLKHVPSGIV VKCHQTRSVDQNRKIARKVL QEKVDVFYNSGPSSG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	469
15N chemical shifts	104
1H chemical shifts	758

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C12orf65	1

Entities:

Entity 1, C12orf65 115 residues - 12305.086 Da.

Residues 1-5 and 110-115 represent a non-native affinity tag.

1

GLY

SER

GLY

SER

GLY

LYS

TRP

GLY

2

LEU

ARG

LEU

GLN

GLU

LYS

PRO

ALA

LEU

3

PHE

PRO

GLY

MET

ALA

SER

THR

VAL

GLN

4

VAL

ALA

GLY

ARG

LYS

ASP

TYR

PRO

ALA

LEU

5

LEU

PRO

LEU

ASN

GLU

SER

GLU

LEU

GLU

6

GLN

PHE

VAL

LYS

GLY

HIS

GLY

PRO

GLY

7

GLN

ALA

THR

ASN

LYS

THR

SER

ASN

CYS

VAL

8

VAL

LEU

LYS

HIS

VAL

PRO

SER

GLY

ILE

VAL

9

VAL

LYS

CYS

HIS

GLN

THR

ARG

SER

VAL

ASP

10

GLN

ASN

ARG

LYS

ILE

ALA

ARG

LYS

VAL

LEU

11

GLN

GLU

LYS

VAL

ASP

VAL

PHE

TYR

ASN

SER

12

GLY

PRO

SER

GLY

Samples:

sample_1: entity, [U-13C; U-15N], 0.96 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, Kobayashi, N. - chemical shift assignment, data analysis

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure solution

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

NMR spectrometers:

Bruker Avance 900 MHz

PDB	2RSM
DBJ	BAB28408 BAC30294 BAE24439
GB	AAH46909 EDL19595 EDL19596
REF	NP_001128189 NP_082586
SP	Q80VP5
AlphaFold	Q80VP5