BMRB Entry 11487

Name: Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Published: 2013-03-12

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PDB ID: 2rv7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11487
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Deposition date:

2012-03-07

Original release date:

2013-03-12

Authors:

Tochio, Naoya; Umehara, Takashi; Kigawa, Takanori; Yokoyama, Shigeyuki

Citation:

Citation: Tochio, Naoya; Umehara, Takashi; Kigawa, Takanori; Yokoyama, Shigeyuki. "Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT" .

Assembly members:

Assembly members:
entity_1, polymer, 92 residues, 10512.312 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: E. coli - cell free Vector: P061010-08

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSSGSSGKPYKCPQCSYASA IKANLNVHLRKHTGEKFACD YCSFTCLSKGHLKVHIERVH KKIKQHCRFCKKKYSDVKNL IKHIRDAHDPQD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	399
15N chemical shifts	83
1H chemical shifts	599

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	ZINC ION_1	2
3	ZINC ION_2	2
4	ZINC ION_3	2

Entities:

Entity 1, entity_1 92 residues - 10512.312 Da.

1

GLY

SER

GLY

SER

GLY

LYS

PRO

TYR

2

LYS

CYS

PRO

GLN

CYS

SER

TYR

ALA

SER

ALA

3

ILE

LYS

ALA

ASN

LEU

ASN

VAL

HIS

LEU

ARG

4

LYS

HIS

THR

GLY

GLU

LYS

PHE

ALA

CYS

ASP

5

TYR

CYS

SER

PHE

THR

CYS

LEU

SER

LYS

GLY

6

HIS

LEU

LYS

VAL

HIS

ILE

GLU

ARG

VAL

HIS

7

LYS

ILE

LYS

GLN

HIS

CYS

ARG

PHE

CYS

8

LYS

TYR

SER

ASP

VAL

LYS

ASN

LEU

9

ILE

LYS

HIS

ILE

ARG

ASP

ALA

HIS

ASP

PRO

10

GLN

ASP

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: protein, [U-13C; U-15N], 0.86 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; zinc chloride 50 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, N. Kobayashi - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Bruker Avance 900 MHz