BMRB Entry 11485

Name: Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Published: 2015-01-20

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PDB ID: 2rv5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11485
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Deposition date:

2012-03-06

Original release date:

2015-01-20

Authors:

Tochio, Naoya; Umehara, Takashi; Kigawa, Takanori; Yokoyama, Shigeyuki

Citation:

Citation: Tochio, Naoya; Umehara, Takashi; Kigawa, Takanori; Yokoyama, Shigeyuki. "Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT" .

Assembly members:

Assembly members:
protein, polymer, 35 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P061010-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
protein: GSSGSSGYVCALCLKKFVSS IRLRSHIREVHGAAQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	136
15N chemical shifts	29
1H chemical shifts	212

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	protein	1
2	ZINC ION	2

Entities:

Entity 1, protein 35 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

TYR

VAL

CYS

2

ALA

LEU

CYS

LEU

LYS

PHE

VAL

SER

3

ILE

ARG

LEU

ARG

SER

HIS

ILE

ARG

GLU

VAL

4

HIS

GLY

ALA

GLN

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: protein, [U-13C; U-15N], 1.10 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; zinc chloride 50 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, N. Kobayashi - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Bruker Avance 800 MHz

PDB	2ELX 2RV5
GB	AAH53930 ABY67968 EDL29394 EDL29395
REF	NP_001139360 NP_941046 XP_006521187 XP_006521188 XP_006521189
SP	Q7TS63
AlphaFold	Q7TS63