BMRB Entry 11480

Name: Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Published: 2015-01-20

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PDB ID: 2rv0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11480
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Deposition date:

2012-03-06

Original release date:

2015-01-20

Authors:

Tochio, Naoya; Umehara, Takashi; Kigawa, Takanori; Yokoyama, Shigeyuki

Citation:

Citation: Tochio, Naoya; Umehara, Takashi; Kigawa, Takanori; Yokoyama, Shigeyuki. "Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT" .

Assembly members:

Assembly members:
protein, polymer, 37 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060718-06

Entity Sequences (FASTA):

Entity Sequences (FASTA):
protein: GSSGSSGRSYSCPVCEKSFS EDRLIKSHIKTNHPEVS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	139
15N chemical shifts	28
1H chemical shifts	207

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	protein	1
2	ZINC ION	2

Entities:

Entity 1, protein 37 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

SER

TYR

2

SER

CYS

PRO

VAL

CYS

GLU

LYS

SER

PHE

SER

3

GLU

ASP

ARG

LEU

ILE

LYS

SER

HIS

ILE

LYS

4

THR

ASN

HIS

PRO

GLU

VAL

SER

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: protein, [U-13C; U-15N], 1.14 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; zinc chloride 50 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, N. Kobayashi - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Bruker Avance 800 MHz

PDB	2ELO 2RV0
DBJ	BAA96009 BAD12567 BAD12568 BAD12569 BAG72872
GB	AAH12989 AAH25423 AAH53930 AAH98405 AAI01767
REF	NP_001025110 NP_001128429 NP_001139360 NP_001161055 NP_001167628
SP	Q7TS63 Q9P243
TPG	DAA22834
AlphaFold	Q7TS63 Q9P243