BMRB Entry 11472

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR11472
MolProbity Validation Chart

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Title:
Complex structure of WWE in RNF146 with ATP
Deposition date:
2012-01-31
Original release date:
2013-03-04
Authors:
He, Fahu; Muto, Yutaka; Inoue, Makoto; Kigawa, Takanori; Shirouzu, Mikako; Terada, Takaho; Yokoyama, Shigeyuki
Citation:

Citation: He, Fahu; Inoue, Makoto; Kigawa, Takanori; Inoue, Mikako; Terada, Takaho; Muto, Yutaka; Yokoyama, Shigeyuki. "Complex structure of WWE domain in RNF146 with ATP"  FEBS Lett. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 110 residues, 12567.119 Da.
ATP, non-polymer, 507.181 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: E. coli - cell free   Vector: P111111

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: PSSGSSGFLDKPTLLSPEEL KAASRGNGEYAWYYEGRNGW WQYDERTSRELEDAFSKGKK NTEMLIAGFLYVADLENMVQ YRRNEHGRRRKIKRDIIDIP KKGVSGPSSG

Data sets:
Data typeCount
13C chemical shifts452
15N chemical shifts105
1H chemical shifts713

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2ADENOSINE-5'-TRIPHOSPHATE2

Entities:

Entity 1, entity_1 110 residues - 12567.119 Da.

1   PROSERSERGLYSERSERGLYPHELEUASP
2   LYSPROTHRLEULEUSERPROGLUGLULEU
3   LYSALAALASERARGGLYASNGLYGLUTYR
4   ALATRPTYRTYRGLUGLYARGASNGLYTRP
5   TRPGLNTYRASPGLUARGTHRSERARGGLU
6   LEUGLUASPALAPHESERLYSGLYLYSLYS
7   ASNTHRGLUMETLEUILEALAGLYPHELEU
8   TYRVALALAASPLEUGLUASNMETVALGLN
9   TYRARGARGASNGLUHISGLYARGARGARG
10   LYSILELYSARGASPILEILEASPILEPRO
11   LYSLYSGLYVALSERGLYPROSERSERGLY

Entity 2, ADENOSINE-5'-TRIPHOSPHATE - C10 H16 N5 O13 P3 - 507.181 Da.

1   ATP

Samples:

sample_1: entity_1, [U-99% 13C; U-99% 15N], 1.0 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - peak picking

Kujira, N. Kobayshi - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAB28015 BAB55108 BAB55196 BAB55359 BAE31549
EMBL CAB66763 CAC85986 CAG38545 CAH89794 CAH90287
GB AAH08235 AAH50795 AAH83675 AAI02140 AIC52443
REF NP_001012060 NP_001070448 NP_001103666 NP_001103667 NP_001103668
SP D2H0Y8 Q2PFU6 Q3T139 Q5REL3 Q5XIK5
TPG DAA26356
AlphaFold D2H0Y8 Q2PFU6 Q3T139 Q5REL3 Q5XIK5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks