BMRB Entry 11468

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11468
MolProbity Validation Chart

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Title:
Solution STRUCTURE OF THE BOMBYX MORI LYSOZYME
Deposition date:
2011-12-19
Original release date:
2013-01-03
Authors:
Sato, Manami; Tochio, Naoya; Aizawa, Tomoyasu
Citation:

Citation: Sato, Manami; Tochio, Naoya; Watanabe, Satoru; Kigawa, Takanori; Aizawa, Tomoyasu. "Solution Structure of the Bombyx Mori LYSOZYME"  .

Assembly members:

Assembly members:
THE BOMBYX MORI LYSOZYME, polymer, 120 residues, 13834.732 Da.

Natural source:

Natural source:   Common Name: domestic silkworm   Taxonomy ID: 7091   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bombyx mori

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P101129-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
THE BOMBYX MORI LYSOZYME: GKTFTRCGLVHELRKHGFEE NLMRNWVCLVEHESSRDTSK TNTNRNGSKDYGLFQINDRY WCSKGASPGKDCNVKCSDLL TDDITKAAKCAKKIYKRHRF DAWYGWKNHCQGSLPDISSC

Data sets:
Data typeCount
13C chemical shifts536
15N chemical shifts139
1H chemical shifts812

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1THE BOMBYX MORI LYSOZYME1

Entities:

Entity 1, THE BOMBYX MORI LYSOZYME 120 residues - 13834.732 Da.

1   GLYLYSTHRPHETHRARGCYSGLYLEUVAL
2   HISGLULEUARGLYSHISGLYPHEGLUGLU
3   ASNLEUMETARGASNTRPVALCYSLEUVAL
4   GLUHISGLUSERSERARGASPTHRSERLYS
5   THRASNTHRASNARGASNGLYSERLYSASP
6   TYRGLYLEUPHEGLNILEASNASPARGTYR
7   TRPCYSSERLYSGLYALASERPROGLYLYS
8   ASPCYSASNVALLYSCYSSERASPLEULEU
9   THRASPASPILETHRLYSALAALALYSCYS
10   ALALYSLYSILETYRLYSARGHISARGPHE
11   ASPALATRPTYRGLYTRPLYSASNHISCYS
12   GLNGLYSERLEUPROASPILESERSERCYS

Samples:

sample_1: sodium acetate 20 mM; sodium chloride 100 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 3.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - structure solution

TOPSPIN, Bruker Biospin - collection

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, Kobayashi, N. - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAB40947
REF NP_001037448
SP P48816
AlphaFold P48816

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks