BMRB Entry 11467

Name: Family GH19 chitinase from Rye seeds
Published: 2012-03-06

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11467

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Family GH19 chitinase from Rye seeds

Deposition date:

2011-12-19

Original release date:

2012-03-06

Authors:

Okazaki, Yoko; Shinya, Shoko; Ohnuma, Takayuki; Fukamizo, Tamo

Citation:

Citation: Okazaki, Yoko; Shinya, Shoko; Ohnuma, Takayuki; Fukamizo, Tamo. "chitinoligosaccharide binding mode of Family GH19 chitinase from rye seeds" Biochemistry ., .-..

Assembly members:

Assembly members:
RSC-c, polymer, 243 residues, Formula weight is not available

Natural source:

Natural source: Common Name: rye Taxonomy ID: 4550 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Secale cereale

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RSC-c: SVSSIISHAQFDRMLLHRND GACQAKGFYTYDAFVAAANA FPGFGATGSTDARKRDVAAF LAQTSHETTGGWATAPDGAF AWGYCFKQERGAAADYCTPS AQWPCAPGKRYYGRGPIQLS HNYNYGPAGRAIGVDLLRNP DLVATDPTVSFKTALWFWMT AQAPKPSSHAVITGKWSPSG ADRAAGRAPGFGVITNIING GLECGHGQDSRVADRIGFYK RYCDILGVGYGDNLDCYNQR PFA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	530
15N chemical shifts	194
1H chemical shifts	194

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Family GH19 chitinase	1

Entities:

Entity 1, Family GH19 chitinase 243 residues - Formula weight is not available

1

SER

VAL

SER

ILE

SER

HIS

ALA

GLN

2

PHE

ASP

ARG

MET

LEU

HIS

ARG

ASN

ASP

3

GLY

ALA

CYS

GLN

ALA

LYS

GLY

PHE

TYR

THR

4

TYR

ASP

ALA

PHE

VAL

ALA

ASN

ALA

5

PHE

PRO

GLY

PHE

GLY

ALA

THR

GLY

SER

THR

6

ASP

ALA

ARG

LYS

ARG

ASP

VAL

ALA

PHE

7

LEU

ALA

GLN

THR

SER

HIS

GLU

THR

GLY

8

GLY

TRP

ALA

THR

ALA

PRO

ASP

GLY

ALA

PHE

9

ALA

TRP

GLY

TYR

CYS

PHE

LYS

GLN

GLU

ARG

10

GLY

ALA

ASP

TYR

CYS

THR

PRO

SER

11

ALA

GLN

TRP

PRO

CYS

ALA

PRO

GLY

LYS

ARG

12

TYR

GLY

ARG

GLY

PRO

ILE

GLN

LEU

SER

13

HIS

ASN

TYR

ASN

TYR

GLY

PRO

ALA

GLY

ARG

14

ALA

ILE

GLY

VAL

ASP

LEU

ARG

ASN

PRO

15

ASP

LEU

VAL

ALA

THR

ASP

PRO

THR

VAL

SER

16

PHE

LYS

THR

ALA

LEU

TRP

PHE

TRP

MET

THR

17

ALA

GLN

ALA

PRO

LYS

PRO

SER

HIS

ALA

18

VAL

ILE

THR

GLY

LYS

TRP

SER

PRO

SER

GLY

19

ALA

ASP

ARG

ALA

GLY

ARG

ALA

PRO

GLY

20

PHE

GLY

VAL

ILE

THR

ASN

ILE

ASN

GLY

21

GLY

LEU

GLU

CYS

GLY

HIS

GLY

GLN

ASP

SER

22

ARG

VAL

ALA

ASP

ARG

ILE

GLY

PHE

TYR

LYS

23

ARG

TYR

CYS

ASP

ILE

LEU

GLY

VAL

GLY

TYR

24

GLY

ASP

ASN

LEU

ASP

CYS

TYR

ASN

GLN

ARG

25

PRO

PHE

ALA

Samples:

sample_1: RSC-c, [U-99% 13C; U-99% 15N], 0.3 mM; sodium acetate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

Bruker Avance 500 MHz

PDB	4DWX 4DYG 4J0L
DBJ	BAB18520
SP	Q9FRV0
AlphaFold	Q9FRV0