BMRB Entry 11458

Name: Solution structure of the N-terminal RNA recognition motif of NonO
Published: 2012-12-18

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PDB ID: 2rs8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11458
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the N-terminal RNA recognition motif of NonO

Deposition date:

2011-11-29

Original release date:

2012-12-18

Authors:

Nagata, Takashi; Muto, Yutaka; Inoue, Makoto; Kigawa, Takanori; Terada, Takaho; Shirouzu, Makiko; Yokoyama, Shigeyuki

Citation:

Citation: Nagata, Takashi. "Solution structure of the N-terminal RNA recognition motif of NonO" .

Assembly members:

Assembly members:
entity, polymer, 99 residues, 10909.508 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: E. coli - cell free Vector: not applicable

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GSSGSSGGEKTFTQRSRLFV GNLPPDITEEEMRKLFEKYG KAGEVFIHKDKGFGFIRLET RTLAEIAKVELDNMPLRGKQ LRVRFACHSASLTSGPSSG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	391
15N chemical shifts	75
1H chemical shifts	625

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N-terminal RNA recognition motif of NonO	1

Entities:

Entity 1, N-terminal RNA recognition motif of NonO 99 residues - 10909.508 Da.

1

GLY

SER

GLY

SER

GLY

GLU

LYS

2

THR

PHE

THR

GLN

ARG

SER

ARG

LEU

PHE

VAL

3

GLY

ASN

LEU

PRO

ASP

ILE

THR

GLU

4

GLU

MET

ARG

LYS

LEU

PHE

GLU

LYS

TYR

GLY

5

LYS

ALA

GLY

GLU

VAL

PHE

ILE

HIS

LYS

ASP

6

LYS

GLY

PHE

GLY

PHE

ILE

ARG

LEU

GLU

THR

7

ARG

THR

LEU

ALA

GLU

ILE

ALA

LYS

VAL

GLU

8

LEU

ASP

ASN

MET

PRO

LEU

ARG

GLY

LYS

GLN

9

LEU

ARG

VAL

ARG

PHE

ALA

CYS

HIS

SER

ALA

10

SER

LEU

THR

SER

GLY

PRO

SER

GLY

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 1.2 mM; TRIS, [U-100% 2H], 20 mM; DTT 1 mM; sodium chloride 100 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

Bruker Avance 700 MHz

PDB	2CPJ 2RS8 3SDE
DBJ	BAB23598 BAB28857 BAC25890 BAE01678 BAE26278
EMBL	CAA72157 CAG32298 CAG33042 CAH89438 CAJ18449
GB	AAA03427 AAB27887 AAC37578 AAC51852 AAH02364
PIR	A54691
REF	NP_001012356 NP_001026703 NP_001040019 NP_001124612 NP_001138880
SP	Q15233 Q5FVM4 Q5RFL9 Q99K48
TPG	DAA12926
AlphaFold	Q15233 Q5FVM4 Q5RFL9 Q99K48