BMRB Entry 11454

Name: NMR Chemical Shift Assignments for QB domain of Sp1
Published: 2012-12-06

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11454

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Chemical Shift Assignments for QB domain of Sp1

Deposition date:

2011-09-26

Original release date:

2012-12-06

Authors:

Hiramatsu, Naoko; Hibino, Emi; Matsuzaki, Katsumi; Kuwahara, Jun; Hoshino, Masaru

Citation:

Citation: Hiramatsu, Naoko; Hibino, Emi; Matsuzaki, Katsumi; Kuwahara, Jun; Hoshino, Masaru. "Interaction between isolated transcriptional activation domains of Sp1 revealed by heteronuclear magnetic resonance" Protein Sci. 21, 1481-1488 (2012).
PubMed: 22855260

Assembly members:

Assembly members:
QB domain of Sp1, polymer, 172 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
QB domain of Sp1: GILTNSQGQTPQRVSGLQGS DALNIQQNQTSGGSLQAGQQ KEGEQNQQTQQQQILIQPQL VQGGQALQALQAAPLSGQTF TTQAISQETLQNLQLQAVPN SGPIIIRTPTVGPNGQVSWQ TLQLQNLQVQNPQAQTITLA PMQGVSLGQTPGGTLEDYKD DDDKGSHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	304
15N chemical shifts	143
1H chemical shifts	143

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	QB domain of Sp1	1

Entities:

Entity 1, QB domain of Sp1 172 residues - Formula weight is not available

1

GLY

ILE

LEU

THR

ASN

SER

GLN

GLY

GLN

THR

2

PRO

GLN

ARG

VAL

SER

GLY

LEU

GLN

GLY

SER

3

ASP

ALA

LEU

ASN

ILE

GLN

ASN

GLN

THR

4

SER

GLY

SER

LEU

GLN

ALA

GLY

GLN

5

LYS

GLU

GLY

GLU

GLN

ASN

GLN

THR

GLN

6

GLN

ILE

LEU

ILE

GLN

PRO

GLN

LEU

7

VAL

GLN

GLY

GLN

ALA

LEU

GLN

ALA

LEU

8

GLN

ALA

PRO

LEU

SER

GLY

GLN

THR

PHE

9

THR

GLN

ALA

ILE

SER

GLN

GLU

THR

LEU

10

GLN

ASN

LEU

GLN

LEU

GLN

ALA

VAL

PRO

ASN

11

SER

GLY

PRO

ILE

ARG

THR

PRO

THR

12

VAL

GLY

PRO

ASN

GLY

GLN

VAL

SER

TRP

GLN

13

THR

LEU

GLN

LEU

GLN

ASN

LEU

GLN

VAL

GLN

14

ASN

PRO

GLN

ALA

GLN

THR

ILE

THR

LEU

ALA

15

PRO

MET

GLN

GLY

VAL

SER

LEU

GLY

GLN

THR

16

PRO

GLY

THR

LEU

GLU

ASP

TYR

LYS

ASP

17

ASP

LYS

GLY

SER

HIS

18

HIS

Samples:

sample_1: QB domain of Sp1, [U-99% 15N], 50 – 550 uM; H2O 90%; D2O 10%; NaCl 140 mM; Na-Phosphate 20 mM

sample_2: QB domain of Sp1, [U-99% 13C; U-99% 15N], 500 uM; H2O 90%; D2O 10%; NaCl 140 mM; Na-Phosphate 20 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.3; pressure: 1 atm; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D hNcocaNH	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMRDraw, Delaglio, Zhengrong and Bax - data analysis

PIPP, Garrett - peak picking

NMR spectrometers:

Bruker DMX 600 MHz

DBJ	BAB13476 BAD92915 BAI46444
EMBL	CBM42955
GB	AAA61154 AAF67726 AAH43224 AAH62539 ADL59934
REF	NP_001238754 NP_001253853 NP_001272689 NP_003100 NP_612482
SP	P08047
AlphaFold	P08047