BMRB Entry 11453

Name: Backbone chemical shifts assignment of UHRF1 atypical PHD finger
Published: 2017-03-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11453

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shifts assignment of UHRF1 atypical PHD finger

Deposition date:

2011-09-22

Original release date:

2017-03-14

Authors:

Qian, Chengmin

Citation:

Citation: Xie, Si; Jakoncic, Jean; Qian, Chengmin. "UHRF1 double tudor domain and the adjacent PHD finger act together to recognize K9me3-containing histone H3 tail" J. Mol. Biol. 415, 318-328 (2012).
PubMed: 22100450

Assembly members:

Assembly members:
PHD_finger, polymer, 74 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PHD_finger: GSPEFGPSCKHCKDDVNRLC RVCACHLCGGRQDPDKQLMC DECDMAFHIYCLDPPLSSVP SEDEWYCPECRNDA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	122
15N chemical shifts	64
1H chemical shifts	64

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PHD finger	1

Entities:

Entity 1, PHD finger 74 residues - Formula weight is not available

1

GLY

SER

PRO

GLU

PHE

GLY

PRO

SER

CYS

LYS

2

HIS

CYS

LYS

ASP

VAL

ASN

ARG

LEU

CYS

3

ARG

VAL

CYS

ALA

CYS

HIS

LEU

CYS

GLY

4

ARG

GLN

ASP

PRO

ASP

LYS

GLN

LEU

MET

CYS

5

ASP

GLU

CYS

ASP

MET

ALA

PHE

HIS

ILE

TYR

6

CYS

LEU

ASP

PRO

LEU

SER

VAL

PRO

7

SER

GLU

ASP

GLU

TRP

TYR

CYS

PRO

GLU

CYS

8

ARG

ASN

ASP

ALA

Samples:

sample_1: PHD finger, [U-13C; U-15N], 0.3 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.150 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks