BMRB Entry 11452

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11452
MolProbity Validation Chart

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Title:
NMR chemical shift assignments for stomagen from Arabidopsis thaliana
Deposition date:
2011-09-01
Original release date:
2012-08-07
Authors:
Ohki, Shinya; Takeuchi, Makoto; Mori, Masashi
Citation:

Citation: Ohki, Shinya; Takeuchi, Makoto; Mori, Masashi. "The NMR structure of stomagen reveals the basis of stomatal density regulation by plant peptide hormones"  Nat. Commun. 2, .-. (2011).
PubMed: 22027592

Assembly members:

Assembly members:
stomagen peptide, polymer, 45 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Nicotiana tabacum   Vector: pBICER8-C0.3-HuIFN-g-SRz

Entity Sequences (FASTA):

Entity Sequences (FASTA):
stomagen peptide: IGSTAPTCTYNECRGCRYKC RAEQVPVEGNDPINSAYHYR CVCHR

Data sets:
Data typeCount
13C chemical shifts153
15N chemical shifts50
1H chemical shifts264

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1stomagen peptide1

Entities:

Entity 1, stomagen peptide 45 residues - Formula weight is not available

1   ILEGLYSERTHRALAPROTHRCYSTHRTYR
2   ASNGLUCYSARGGLYCYSARGTYRLYSCYS
3   ARGALAGLUGLNVALPROVALGLUGLYASN
4   ASPPROILEASNSERALATYRHISTYRARG
5   CYSVALCYSHISARG

Samples:

sample_1: Stomagen0.5 – 0.6 mM; sodium phosphate 10 mM; potassium chloride 100 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_2: Stomagen0.5 – 0.6 mM; sodium phosphate 10 mM; potassium chloride 100 mM; sodium azide 0.02%; D2O 100%

sample_3: Stomagen, [U-15N], 0.1 – 0.2 mM; sodium phosphate 10 mM; potassium chloride 100 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_4: Stomagen, [U-13C; U-15N], 0.3 – 0.4 mM; sodium phosphate 10 mM; potassium chloride 100 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESY no.1sample_1isotropicsample_conditions_1
2D 1H-1H NOESY no.2sample_2isotropicsample_conditions_1
2D 1H-1H TOCSY no.1sample_1isotropicsample_conditions_1
2D 1H-1H TOCSY no.2sample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D HNCOsample_4isotropicsample_conditions_1
3D HNCAsample_4isotropicsample_conditions_1
3D CBCA(CO)NHsample_4isotropicsample_conditions_1
3D HNCACBsample_4isotropicsample_conditions_1
3D HCCH-TOCSYsample_4isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

SPARKY, Goddard - chemical shift assignment, peak picking

X-PLOR NIH v2.26, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAD43639
EMBL CAB45496 CAB78339 CDX80700 CDY34535
GB AEE83209 EFH39459 EOA17576 ESQ56436 KGN44656
REF NP_193033 XP_002863200 XP_004290145 XP_006284678 XP_006414983
SP Q9SV72
AlphaFold Q9SV72

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks