BMRB Entry 11442

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11442

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Title:
Backbone 1H, 13C and 15N chemical shift assignments of Hd3a (K31A/E57A)
Deposition date:
2011-05-20
Original release date:
2011-08-17
Authors:
Taoka, Ken-ichiro; Ohki, Izuru; Tsuji, Hiroyuki; Furuita, Kyoko; Hayashi, Kokoro; Yanase, Tomoko; Yamaguchi, Midori; Nakashima, Chika; Purwestri, Yekti; Tamaki, Shojiro; Ogaki, Yuka; Shimada, Chihiro; Nakagawa, Atsushi; Kojima, Chojiro; Shimamoto, Ko
Citation:

Citation: Taoka, Ken-ichiro; Ohki, Izuru; Tsuji, Hiroyuki; Furuita, Kyoko; Hayashi, Kokoro; Yanase, Tomoko; Yamaguchi, Midori; Nakashima, Chika; Purwestri, Yekti; Tamaki, Shojiro; Ogaki, Yuka; Shimada, Chihiro; Nakagawa, Atsushi; Kojima, Chojiro; Shimamoto, Ko. "14-3-3 proteins act as intracellular receptors for rice Hd3a florigen"  Nature 476, 332-335 (2011).
PubMed: 21804566

Assembly members:

Assembly members:
Hd3a_(K31A/E57A), polymer, 183 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: rice   Taxonomy ID: 4530   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Oryza sativa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCold-GST

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hd3a_(K31A/E57A): GPGHMAGSGRDRDPLVVGRV VGDVLDAFVRSTNLAVTYGS KTVSNGCELKPSMVTHQPRV AVGGNDMRTFYTLVMVDPDA PSPSDPNLREYLHWLVTDIP GTTAASFGQEVMCYESPRPT MGIHRLVFVLFQQLGRQTVY APGWRQNFNTKDFAELYNLG SPVAAVYFNCQREAGSGGRR VYP

Data sets:
Data typeCount
13C chemical shifts441
15N chemical shifts140
1H chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hd3a (K31A/E57A)1

Entities:

Entity 1, Hd3a (K31A/E57A) 183 residues - Formula weight is not available

1   GLYPROGLYHISMETALAGLYSERGLYARG
2   ASPARGASPPROLEUVALVALGLYARGVAL
3   VALGLYASPVALLEUASPALAPHEVALARG
4   SERTHRASNLEUALAVALTHRTYRGLYSER
5   LYSTHRVALSERASNGLYCYSGLULEULYS
6   PROSERMETVALTHRHISGLNPROARGVAL
7   ALAVALGLYGLYASNASPMETARGTHRPHE
8   TYRTHRLEUVALMETVALASPPROASPALA
9   PROSERPROSERASPPROASNLEUARGGLU
10   TYRLEUHISTRPLEUVALTHRASPILEPRO
11   GLYTHRTHRALAALASERPHEGLYGLNGLU
12   VALMETCYSTYRGLUSERPROARGPROTHR
13   METGLYILEHISARGLEUVALPHEVALLEU
14   PHEGLNGLNLEUGLYARGGLNTHRVALTYR
15   ALAPROGLYTRPARGGLNASNPHEASNTHR
16   LYSASPPHEALAGLULEUTYRASNLEUGLY
17   SERPROVALALAALAVALTYRPHEASNCYS
18   GLNARGGLUALAGLYSERGLYGLYARGARG
19   VALTYRPRO

Samples:

sample_1: Hd3a (K31A/E57A), [U-13C; U-15N], 0.2 mM; potassium phosphate 50 mM; potassium chloride 100 mM; DTT 1 mM; H2O 93%; D2O 7%

sample_conditions_1: pH: 6.8; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

RNMRTK, Jeff Hoch and Alan Stern - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

DBJ BAB61027 BAB61028 BAB61029 BAB61030 BAC21280
GB ABB17666 ABB17667 AFK31055 AFK31056 AFK31057
REF NP_001056860
SP Q93WI9
AlphaFold Q93WI9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks