BMRB Entry 11436

Name: NMR Chemical Shift Assignments for E73A mutant of Ostrich egg white Lysozyme
Published: 2011-09-01

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11436

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Chemical Shift Assignments for E73A mutant of Ostrich egg white Lysozyme

Deposition date:

2011-03-18

Original release date:

2011-09-01

Authors:

Shinya, Shoko; Ohnuma, Takayuki; Kawamura, Shunsuke; Nishimura, Shigenori; Fukamizo, Tamo

Citation:

Citation: Shinya, Shoko; Ohnuma, Takayuki; Kawamura, Shunsuke; Torikata, Takao; Nishimura, Shigenori; Katoh, Etsuko; Fukamizo, Tamo. "Interaction of a goose-type lysozyme with chitin oligosaccharides as determined by NMR spectroscopy" J. Biochem. 150, 569-577 (2011).
PubMed: 21859795

Assembly members:

Assembly members:
E73A-OEL, polymer, 187 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Ostrich Taxonomy ID: 8801 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Struthio Linnaeus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: pPIC9K

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E73A-OEL: SRTGCYGDVNRVDTTGASCK SAKPEKLNYCGVAASRKIAE RDLQSMDRYKALIKKVGQKL CVDPAVIAGIISRASHAGKA LRNGWGDNGNGFGLMQVDRR SHKPVGEWNGERHLMQGTEI LISMIKAIQKKFPRWTKEQQ LKGGISAYNAGPGNVRSYER MDIGTTHDDYANDVVARAQY YKQHGYX

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	339
15N chemical shifts	174
1H chemical shifts	174

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	E73A mutant ofOstrich egg white lysozyme	1

Entities:

Entity 1, E73A mutant ofOstrich egg white lysozyme 187 residues - Formula weight is not available

1

SER

ARG

THR

GLY

CYS

TYR

GLY

ASP

VAL

ASN

2

ARG

VAL

ASP

THR

GLY

ALA

SER

CYS

LYS

3

SER

ALA

LYS

PRO

GLU

LYS

LEU

ASN

TYR

CYS

4

GLY

VAL

ALA

SER

ARG

LYS

ILE

ALA

GLU

5

ARG

ASP

LEU

GLN

SER

MET

ASP

ARG

TYR

LYS

6

ALA

LEU

ILE

LYS

VAL

GLY

GLN

LYS

LEU

7

CYS

VAL

ASP

PRO

ALA

VAL

ILE

ALA

GLY

ILE

8

ILE

SER

ARG

ALA

SER

HIS

ALA

GLY

LYS

ALA

9

LEU

ARG

ASN

GLY

TRP

GLY

ASP

ASN

GLY

ASN

10

GLY

PHE

GLY

LEU

MET

GLN

VAL

ASP

ARG

11

SER

HIS

LYS

PRO

VAL

GLY

GLU

TRP

ASN

GLY

12

GLU

ARG

HIS

LEU

MET

GLN

GLY

THR

GLU

ILE

13

LEU

ILE

SER

MET

ILE

LYS

ALA

ILE

GLN

LYS

14

LYS

PHE

PRO

ARG

TRP

THR

LYS

GLU

GLN

15

LEU

LYS

GLY

ILE

SER

ALA

TYR

ASN

ALA

16

GLY

PRO

GLY

ASN

VAL

ARG

SER

TYR

GLU

ARG

17

MET

ASP

ILE

GLY

THR

HIS

ASP

TYR

18

ALA

ASN

ASP

VAL

ALA

ARG

ALA

GLN

TYR

19

TYR

LYS

GLN

HIS

GLY

TYR

205

Samples:

sample_1: E73A-OEL, [U-99% 13C; U-99% 15N], 0.4 mM; sodium acetate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz

BMRB	11434
PDB	3WYH
DBJ	BAL03620
GB	KFV76629
REF	XP_009664638
SP	P00719
AlphaFold	P00719