BMRB Entry 11430

Name: SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF THE HMGB2
Published: 2012-06-05

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11430

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF THE HMGB2

Deposition date:

2011-02-23

Original release date:

2012-06-05

Authors:

KURITA, J.; UEWAKI, J.; SHIMAHARA, H.; YOSHIDA, M.; TATE, S.

Citation:

Citation: KURITA, J.; UEWAKI, J.; SHIMAHARA, H.; YOSHIDA, M.; TATE, S.. "STRUCUTRAL COMPARISON OF TWO HMG-BOXES IN THE NON-HISTONE PROTEIN HMG-2 WITH IN THE HMG-1" .

Assembly members:

Assembly members:
HMG2B, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source: Common Name: pig Taxonomy ID: 9823 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Sus scrofa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HMG2B: MKKDPNAPKRPPSAFFLFCS EHRPKIKSEHPGLSIGDTAK KLGEMWSEQSAKDKQPYEQK AAKLKEKYEKDIAAYRAK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	276
15N chemical shifts	75
1H chemical shifts	544

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HMG2B	1

Entities:

Entity 1, HMG2B 78 residues - Formula weight is not available

1

MET

LYS

ASP

PRO

ASN

ALA

PRO

LYS

ARG

2

PRO

SER

ALA

PHE

LEU

PHE

CYS

SER

3

GLU

HIS

ARG

PRO

LYS

ILE

LYS

SER

GLU

HIS

4

PRO

GLY

LEU

SER

ILE

GLY

ASP

THR

ALA

LYS

5

LYS

LEU

GLY

GLU

MET

TRP

SER

GLU

GLN

SER

6

ALA

LYS

ASP

LYS

GLN

PRO

TYR

GLU

GLN

LYS

7

ALA

LYS

LEU

LYS

GLU

LYS

TYR

GLU

LYS

8

ASP

ILE

ALA

TYR

ARG

ALA

LYS

Samples:

sample_1: HMG2B, [U-13C; U-15N], 1 mM; H2O 95%; D2O 5%; sodium acetate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

NMR spectrometers:

Varian INOVA 750 MHz

BMRB	11431
PDB	1J3C 1J3D
EMBL	CAA78938
GB	AAA48819 EDL28622 EDM11073 EGV91758 ELW73033
REF	XP_002187513 XP_003938773 XP_004610441 XP_006022293 XP_006194086
TPG	DAA30815