BMRB Entry 11430

Title:
SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF THE HMGB2
Deposition date:
2011-02-23
Original release date:
2012-06-05
Authors:
KURITA, J.; UEWAKI, J.; SHIMAHARA, H.; YOSHIDA, M.; TATE, S.
Citation:

Citation: KURITA, J.; UEWAKI, J.; SHIMAHARA, H.; YOSHIDA, M.; TATE, S.. "STRUCUTRAL COMPARISON OF TWO HMG-BOXES IN THE NON-HISTONE PROTEIN HMG-2 WITH IN THE HMG-1"  .

Assembly members:

Assembly members:
HMG2B, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: pig   Taxonomy ID: 9823   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Sus scrofa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts276
15N chemical shifts75
1H chemical shifts544

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HMG2B1

Entities:

Entity 1, HMG2B 78 residues - Formula weight is not available

1   METLYSLYSASPPROASNALAPROLYSARG
2   PROPROSERALAPHEPHELEUPHECYSSER
3   GLUHISARGPROLYSILELYSSERGLUHIS
4   PROGLYLEUSERILEGLYASPTHRALALYS
5   LYSLEUGLYGLUMETTRPSERGLUGLNSER
6   ALALYSASPLYSGLNPROTYRGLUGLNLYS
7   ALAALALYSLEULYSGLULYSTYRGLULYS
8   ASPILEALAALATYRARGALALYS

Samples:

sample_1: HMG2B, [U-13C; U-15N], 1 mM; H2O 95%; D2O 5%; sodium acetate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

BMRB 11431
PDB
EMBL CAA78938
GB AAA48819 EDL28622 EDM11073 EGV91758 ELW73033
REF XP_002187513 XP_003938773 XP_004610441 XP_006022293 XP_006194086
TPG DAA30815

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks