BMRB Entry 11429

Name: SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF THE HMGB2
Published: 2012-06-05

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11429

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF THE HMGB2

Deposition date:

2011-02-22

Original release date:

2012-06-05

Authors:

KURITA, J.; UEWAKI, J.; SHIMAHARA, H.; YOSHIDA, M.; TATE, S.

Citation:

Citation: KURITA, J.; UEWAKI, J.; SHIMAHARA, H.; YOSHIDA, M.; TATE, S.. "STRUCUTRAL COMPARISON OF TWO HMG-BOXES IN THE NON-HISTONE PROTEIN HMG-2 WITH IN THE HMG-1" .

Assembly members:

Assembly members:
HMG2A, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source: Common Name: pig Taxonomy ID: 9823 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Sus scrofa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HMG2A: MGKGDPNKPRGKMSSYAFFV QTSREEHKKKHPDSSVNFAE FSKKCSERWKTMSAKEKSKF EDMAKSDKARYDREMKN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	220
15N chemical shifts	77
1H chemical shifts	483

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HMG2A	1

Entities:

Entity 1, HMG2A 77 residues - Formula weight is not available

1

MET

GLY

LYS

GLY

ASP

PRO

ASN

LYS

PRO

ARG

2

GLY

LYS

MET

SER

TYR

ALA

PHE

VAL

3

GLN

THR

SER

ARG

GLU

HIS

LYS

4

HIS

PRO

ASP

SER

VAL

ASN

PHE

ALA

GLU

5

PHE

SER

LYS

CYS

SER

GLU

ARG

TRP

LYS

6

THR

MET

SER

ALA

LYS

GLU

LYS

SER

LYS

PHE

7

GLU

ASP

MET

ALA

LYS

SER

ASP

LYS

ALA

ARG

8

TYR

ASP

ARG

GLU

MET

LYS

ASN

Samples:

sample_1: HMG2A, [U-13C; U-15N], 1 mM; H2O 95%; D2O 5%; sodium acetate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

NMR spectrometers:

Varian INOVA 750 MHz

PDB	1J3X
REF	XP_006040222 XP_007083590 XP_008582451 XP_008582452 XP_008582454