BMRB Entry 11425

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11425

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Title:
1H, 13C, and 15N resonance assignment of the SPFH domain of human stomatin.
Deposition date:
2011-01-15
Original release date:
2011-06-21
Authors:
Tsuruta, Tomoyuki; Goda, Natsuko; Umetsu, Yoshitaka; Iwaya, Naoko; Kuwahara, Yohta; Hiroaki, Hidekazu
Citation:

Citation: Tsuruta, Tomoyuki; Goda, Natsuko; Umetsu, Yoshitaka; Iwaya, Naoko; Kuwahara, Yohta; Hiroaki, Hidekazu. "(1)H, (13)C, and (15)N resonance assignment of the SPFH domain of human stomatin."  Biomol. NMR Assignments ., .-. (2011).
PubMed: 21643969

Assembly members:

Assembly members:
SPFH domain of human stomatin, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SPFH domain of human stomatin: GSDHVDMRTISFDIPPQEIL TKDSVTISVDGVVYYRVQNA TLAVANITNADSATRLLAQT TLRNVLGTKNLSQILSDREE IAHNMQSTLDDATDAWGIKV ERVEIKDVKLPVQ

Data sets:
Data typeCount
13C chemical shifts453
15N chemical shifts122
1H chemical shifts763

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SPFH domain of human stomatin1

Entities:

Entity 1, SPFH domain of human stomatin 113 residues - Formula weight is not available

Residues 1-4 represent a non-native affinity tag. This is SPFH domain of human stomatin (residues 94 202).

1   GLYSERASPHISVALASPMETARGTHRILE
2   SERPHEASPILEPROPROGLNGLUILELEU
3   THRLYSASPSERVALTHRILESERVALASP
4   GLYVALVALTYRTYRARGVALGLNASNALA
5   THRLEUALAVALALAASNILETHRASNALA
6   ASPSERALATHRARGLEULEUALAGLNTHR
7   THRLEUARGASNVALLEUGLYTHRLYSASN
8   LEUSERGLNILELEUSERASPARGGLUGLU
9   ILEALAHISASNMETGLNSERTHRLEUASP
10   ASPALATHRASPALATRPGLYILELYSVAL
11   GLUARGVALGLUILELYSASPVALLYSLEU
12   PROVALGLN

Samples:

sample_1: SPFH domain of human stomatin, [U-13C; U-15N], 0.6 mM; sodium phosphate 50 mM; sodium chloride 100 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

NMRPipe v5.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.110, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III 600 MHz

Related Database Links:

DBJ BAG64120 BAG65267 BAG82675 BAG82676 BAI46539
EMBL CAA42671 CAA59436 CAG46897
GB AAA58432 AAC50296 AAH10703 AAI51432 AAX36989
PRF 2201444A 2204264A
REF NP_001098943 NP_001136142 NP_001253158 NP_001257455 NP_004090
SP P27105
TPG DAA26426
AlphaFold P27105

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks