BMRB Entry 11417

Name: Solution structure of the J domain of DnaJ homolog subfamily B member 8
Published: 2010-10-08

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PDB ID: 2dmx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11417
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the J domain of DnaJ homolog subfamily B member 8

Deposition date:

2010-09-24

Original release date:

2010-10-08

Authors:

Ohnishi, S.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Ohnishi, S.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the J domain of DnaJ homolog subfamily B member 8" .

Assembly members:

Assembly members:
DnaJ domain, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050613-27

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DnaJ domain: GSSGSSGMANYYEVLGVQAS ASPEDIKKAYRKLALRWHPD KNPDNKEEAEKKFKLVSEAY EVLSDSKKRSLYDRAGCDSW RAGGGASGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	373
15N chemical shifts	87
1H chemical shifts	554

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DnaJ domain	1

Entities:

Entity 1, DnaJ domain 92 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

ALA

ASN

2

TYR

GLU

VAL

LEU

GLY

VAL

GLN

ALA

SER

3

ALA

SER

PRO

GLU

ASP

ILE

LYS

ALA

TYR

4

ARG

LYS

LEU

ALA

LEU

ARG

TRP

HIS

PRO

ASP

5

LYS

ASN

PRO

ASP

ASN

LYS

GLU

ALA

GLU

6

LYS

PHE

LYS

LEU

VAL

SER

GLU

ALA

TYR

7

GLU

VAL

LEU

SER

ASP

SER

LYS

ARG

SER

8

LEU

TYR

ASP

ARG

ALA

GLY

CYS

ASP

SER

TRP

9

ARG

ALA

GLY

ALA

SER

GLY

PRO

SER

10

SER

GLY

Samples:

sample_1: DnaJ domain, [U-13C; U-15N], 1.3 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.965, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz

PDB	2DMX
DBJ	BAE01188 BAG54269 BAG54285
GB	AAH29521 AAH50288 ADQ32829 AIC53322 EAW79320
REF	NP_001271507 NP_699161 XP_001097989 XP_005979495 XP_006713582
SP	Q8NHS0
AlphaFold	Q8NHS0