BMRB Entry 11416

Name: Solution structure of C2H2 type Zinc finger domain 5 in Zinc finger protein 32
Published: 2011-09-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11416

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of C2H2 type Zinc finger domain 5 in Zinc finger protein 32

Deposition date:

2010-09-09

Original release date:

2011-09-08

Authors:

Kasahara, N.; Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation:

Citation: Kasahara, N.; Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of C2H2 type Zinc finger domain 5 in Zinc finger protein 32" .

Assembly members:

Assembly members:
zinc-finger motif, polymer, 42 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P061204-06

Entity Sequences (FASTA):

Entity Sequences (FASTA):
zinc-finger motif: GSSGSSGGEKPYRCDQCGKA FSQKGSLIVHIRVHTGSGPS SG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	137
15N chemical shifts	31
1H chemical shifts	214

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	zinc-finger motif	1
2	ZINC ION	2

Entities:

Entity 1, zinc-finger motif 42 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

LYS

2

PRO

TYR

ARG

CYS

ASP

GLN

CYS

GLY

LYS

ALA

3

PHE

SER

GLN

LYS

GLY

SER

LEU

ILE

VAL

HIS

4

ILE

ARG

VAL

HIS

THR

GLY

SER

GLY

PRO

SER

5

SER

GLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: zinc-finger motif mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060702, Delaglio F. - processing

NMRView v5.0.4, Jonson B.A. - data analysis

Kujira v0.9820, Kobayashi N. - data analysis

CYANA v2.0.17, Gutert P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz