BMRB Entry 11414

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11414

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of C2H2 type Zinc finger domain 345 in Zinc finger protein 278

Deposition date:

2010-09-09

Original release date:

2011-09-08

Authors:

Kasahara, N.; Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation:

Citation: Kasahara, N.; Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of C2H2 type Zinc finger domain 345 in Zinc finger protein 278" .

Assembly members:

Assembly members:
C2H2 domain, polymer, 95 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060718-20

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C2H2 domain: GSSGSSGVACEICGKIFRDV YHLNRHKLSHSGEKPYSCPV CGLRFKRKDRMSYHVRSHDG SVGKPYICQSCGKGFSRPDH LNGHIKQVHSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	376
15N chemical shifts	83
1H chemical shifts	579

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C2H2 domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2
4	ZINC ION no.3	2

Entities:

Entity 1, C2H2 domain 95 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

VAL

ALA

CYS

2

GLU

ILE

CYS

GLY

LYS

ILE

PHE

ARG

ASP

VAL

3

TYR

HIS

LEU

ASN

ARG

HIS

LYS

LEU

SER

HIS

4

SER

GLY

GLU

LYS

PRO

TYR

SER

CYS

PRO

VAL

5

CYS

GLY

LEU

ARG

PHE

LYS

ARG

LYS

ASP

ARG

6

MET

SER

TYR

HIS

VAL

ARG

SER

HIS

ASP

GLY

7

SER

VAL

GLY

LYS

PRO

TYR

ILE

CYS

GLN

SER

8

CYS

GLY

LYS

GLY

PHE

SER

ARG

PRO

ASP

HIS

9

LEU

ASN

GLY

HIS

ILE

LYS

GLN

VAL

HIS

SER

10

GLY

PRO

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: C2H2 domain mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060702, Delaglio F. - processing

NMRView v5.0.4, Jonson B.A. - data analysis

Kujira v0.9820, Kobayashi N. - data analysis

CYANA v2.0.17, Gutert P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2YT9
DBJ	BAE38619 BAF84492
GB	AAF32518 AAF99602 AAG09031 AAG09035 AAH21091
REF	NP_001240620 NP_001264144 NP_114440 XP_001927041 XP_003317256